抗Human Lgr5/GPR49抗体(Anti-Human Lgr5/GPR49 antibody)
掲載日情報：2018/11/26 現在Webページ番号：196659
Human Lgr5/GPR49に対する抗体(Anti-Human Lgr5/GPR49 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月04日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Human Lgr5/GPR49 MAb (Clone 707042) |
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Anti-Human Lgr5/GPR49 MAb (Clone 707042) |
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[在庫・価格 ：2024年05月04日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human Lgr5/GPR49 MAb (Clone 707042)
文献数： 2
- 商品コード：MAB8078
- メーカー：RSD
- 包装：100μg
- 価格：¥104,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
別名：FEX クローン：707042 Genbank No： 8549 Protein Accession No： O75473 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | FCM,IC | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
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| 製品記事 |
肺オルガノイド培養関連製品（Products for Lung Organoids） |
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Anti-Human Lgr5/GPR49 MAb (Clone 707042)
文献数： 1
- 商品コード：MAB8078-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。形状：溶液または凍結乾燥 別名：FEX クローン：707042 Genbank No： 8549 Protein Accession No： O75473 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | FCM,IC | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
肺オルガノイド培養関連製品（Products for Lung Organoids） |
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| 関連記事 | |||||||
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human Lgr5/GPR49Met1-Ile560Accession # O75473 |
| Source | Monoclonal Mouse IgG2A Clone # 707042 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human Lgr5/GPR49 in ELISA. Stains human and mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD42", has been found to detect an epitope in C-terminal LRR cap of LGR5 (Ref. 1). |
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Applications and Data
| Recommended Concentration | Sample | |
| Flow Cytometry | 2.5 µg/106 cells | See below |
| Agonist Activity | Peng, W.C., et al. (2013) Cell Reports. 3:1885. | |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Flow Cytometry | |
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 | Detection of Lgr5/GPR49 in NS0 Mouse Cell Line Transfected with Human Lgr5/GPR49 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human Lgr5/GPR49 and eGFP was stained with and either (A) Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). |
| Immunocytochemistry | |
 | Lgr5/GPR49 in NSO Mouse Cell Line Transfected with Human Lgr5/GPR49. Lgr5/GPR49 was detected in immersion fixed NSO mouse myeloma cell line transfected with GFP (green) tagged human LGR5 using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Immunocytochemistry | |
 | Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49. Lgr5/GPR49 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with GFP (green) tagged mouse LGR5 using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Immunocytochemistry | |
 | Lgr5/GPR49 in Human Cells Transfected with Human Lgr5/GPR49. Lgr5/GPR49 was detected in MYC tagged human transfectants using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078). Cells were stained using an anti-mouse HRP-conjugated secondary antibody (brown). Specific staining was localized to cytoplasm. Image courtesy of Dr. Hans Clevers, Hubrecht Institute, The Netherlands. |
| Immunocytochemistry | |
 | Lgr5/GPR49 in Mouse Cells Transfected with Mouse Lgr5/GPR49. Lgr5/GPR49 was detected in immersion fixed MYC tagged mouse transfectants using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078). Cells were stained using an anti-mouse HRP-conjugated secondary antibody (brown). Specific staining was localized to cytoplasm. Image courtesy of Dr. Hans Clevers, Hubrecht Institute, The Netherlands. |
| Agonist Activity | |
 | Human Lgr5/GPR49 Antibody Induces Activity. Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) induces TOPflash activity in the HEK293 human embryonic kidney cell line stably expressing LGR5 in the presence of Wnt-3a, but in the absence of R-Spondins. (Data courtesy of Dr. Wim de Lau and Dr. Hans Clevers, Hubrecht Institute, The Netherlands. See Reference 1.) |
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Related Product & Information
| Background | Lgr5/GPR49 |
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| background_content | Background: Lgr5/GPR49 Leucine‑rich repeat G‑protein‑coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the human Lgr5 long extracellular domain used as an immunogen shares 90% amino acid sequence identity with mouse and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5+ stem cells can produce all epithelial cell types of the intestinal crypts (4). Abnormal LGR5 expression and regulation in stem cells might give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD42" in Peng et al. (1). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- Musashi-1 promotes a cancer stem cell lineage and chemoresistance in colorectal cancer cells
Authors: GY Chiou, TW Yang, CC Huang, CY Tang, JY Yen, MC Tsai, HY Chen, N Fadhilah, CC Lin, YJ Jong
Sci Rep, 2017;7(1):2172.
Species: Human
Sample Type: Cell Lysates
Application: WB
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