抗Human gp96/HSP90B1抗体(Anti-Human gp96/HSP90B1 antibody)
掲載日情報：2018/11/26 現在Webページ番号：196595
Human gp96/HSP90B1に対する抗体(Anti-Human gp96/HSP90B1 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月06日 14時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Human gp96/HSP90B1 MAb (Clone 816803) |
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Anti-Human gp96/HSP90B1 MAb (Clone 816803) |
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[在庫・価格 ：2024年05月06日 14時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human gp96/HSP90B1 MAb (Clone 816803)
文献数： 1
- 商品コード：MAB7606
- メーカー：RSD
- 包装：100μg
- 価格：¥94,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
Simple Western対応抗体。 別名：94 kDa glucose-regulated protein クローン：816803 Genbank No： 7184 Protein Accession No： P14625 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | IC,IHC,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
Mouse Anti-Human IL-17F Monoclonal Antibody / Mouse Anti-Human gp96/HSP90B1 Monoclonal Antibody |
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Anti-Human gp96/HSP90B1 MAb (Clone 816803)
文献数： 1
- 商品コード：MAB7606-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
Simple Western対応抗体。※受注発注品。形状：溶液または凍結乾燥 別名：94 kDa glucose-regulated protein クローン：816803 Genbank No： 7184 Protein Accession No： P14625 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | IC,IHC,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human gp96/HSP90B1Arg503-Arg660Accession # P14625 |
| Source | Monoclonal Mouse IgG2A Clone # 816803 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human gp96/HSP90B1 in direct ELISAs and Western blots. Detects mouse and rat gp96/HSP90B1 in Western blots. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.2 µg/mL | See below |
| Simple Western | 2 µg/mL | See below |
| Immunohistochemistry | 8-25 µg/mL | See below |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Knockout Validated | Gp96/HSP90B1 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in gp96/HSP90B1 knockout HEK293T cell line. | |
| Western Blot | |
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 | Detection of Human, Mouse, and Rat gp96/HSP90B1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, CH‑1 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human gp96/HSP90B1 Monoclonal Antibody (Catalog # MAB7606) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for gp96/HSP90B1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunocytochemistry | |
 | gp96/HSP90B1 in HeLa Human Cell Line. gp96/HSP90B1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human gp96/HSP90B1 Monoclonal Antibody (Catalog # MAB7606) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Immunohistochemistry | |
 | gp96/HSP90B1 in Human Mesothelioma. gp96/HSP90B1 was detected in immersion fixed paraffin-embedded sections of human mesothelioma using Mouse Anti-Human gp96/HSP90B1 Monoclonal Antibody (Catalog # MAB7606) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
| Simple Western | |
 | Detection of Human gp96/HSP90B1 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for gp96/HSP90B1 at approximately 108 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human gp96/HSP90B1 Monoclonal Antibody (Catalog # MAB7606). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
| Knockout Validated | |
 | Western Blot Shows Human gp96/HSP90B1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and gp96/HSP90B1 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human gp96/HSP90B1 Monoclonal Antibody (Catalog # MAB7606) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for gp96/HSP90B1 at approximately 100 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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Related Product & Information
| Long Name | Heat Shock Protein 90 beta 1 |
|---|---|
| Background | gp96/HSP90B1 |
| background_content | Background: gp96/HSP90B1 Glycoprotein 96 (gp96; also endoplasmin, GRP-94, TRA1 and HSP90B1) is a 94-100 kDa member of the HSP 90 family of proteins. gp96 is a ubiquitously-expressed ER resident protein that is found in a preformed complex with BiP, CaBP1 and UDP-glucosyltransferase. This is a chaperone complex that binds unfolded protein substrates. When folded properly, the substrate is forwarded to calnexin-containing chaperone complexes that promote its maturation. gp96 clients are restricted and include disulfide-bonded integrins, TLRs, LDLR and CD180. Within the complex, gp96 exists as a disulfide-linked homodimer that may form higher-order oligomers. gp96 also appears on the cell surface and may serve as a receptor for bacteria. Mature human gp96 is a 782 amino acid (aa) membrane-associated protein(aa 22-803). It is not a transmembrane protein but utilizes an ER retention signal (aa 800-803) to interact with the ER membrane. The molecule possesses aHATPase-C like region (aa 98-219) plus multiple ATP binding and two utilized phosphorylation sites. Over aa 503-660, human gp96 shares 98% aa sequence identity with mouse gp96. |
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