抗Mouse EDG-1/S1P1抗体(Anti-Mouse EDG-1/S1P1 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)Accession # O08530
Source	Monoclonal Rat IgG2A Clone # 713412
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse S1P1/EDG‑1 peptide in direct ELISAs.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
Immunohistochemistry	8-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry
	Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected With S1P1/EDG‑1 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG‑1 and GFP was stained with (A) Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A isotype control antibody (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
Flow Cytometry
	Detection of S1P1/EDG‑1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
Immunohistochemistry
	S1P1/EDG‑1 in Mouse Heart. S1P1/EDG‑1 was detected in perfusion fixed frozen sections of mouse heart using Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter­stained with hematoxylin (blue). Specific staining was localized to endothelial cells in capillaries. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Long Name	Endothelial Differentiation Gene 1
Background	S1P1/EDG-1
background_content	Background: S1P1/EDG-1 S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.

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Citations

1. Macrophages Induce Long-Term Trapping of ?? T Cells with Innate-like Properties within Secondary Lymphoid Organs in the Steady State
   Authors: A Audemard-V, M Rivière, A Durand, E Peranzoni, V Guichard, P Hamon, N Bonilla, T Guilbert, A Boissonnas, C Auffray, G Eberl, B Lucas, B Martin
   J. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. A Coronin 1-Dependent Decision Switch in Juvenile Mice Determines the Population of the Peripheral Naive T Cell Compartment
   Authors: MJ Lang, M Mori, J Ruer-Laven, J Pieters
   J. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. Lymphatic endothelial S1P promotes mitochondrial function and survival in naive T cells
   Authors: A Mendoza, V Fang, C Chen, M Serasinghe, A Verma, J Muller, VS Chaluvadi, ML Dustin, T Hla, O Elemento, JE Chipuk, SR Schwab
   Nature, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. CD8?? intraepithelial lymphocytes arise from two main thymic precursors
   Authors: R Ruscher, RL Kummer, YJ Lee, SC Jameson, KA Hogquist
   Nat. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Ageing adversely affects the migration and function of marginal zone B cells
   Authors: VM Turner, NA Mabbott
   Immunology, 2017;151(3):349-362.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


6. Mir-17-92 regulates bone marrow homing of plasma cells and production of immunoglobulin G2c.
   Authors: Xu, Shengli, Ou, Xijun, Huo, Jianxin, Lim, Kristen, Huang, Yuhan, Chee, Sheena, Lam, Kong-Pen
   Nat Commun, 2015;6(0):6764.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


7. IL-17-Producing Vgamma4+ gammadelta T Cells Require Sphingosine 1-Phosphate Receptor 1 for Their Egress from the Lymph Nodes under Homeostatic and Inflammatory Conditions.
   Authors: Maeda Y, Seki N, Kataoka H, Takemoto K, Utsumi H, Fukunari A, Sugahara K, Chiba K
   J Immunol, 2015;195(4):1408-16.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


8. Control of lymphocyte egress from lymph nodes through beta2-adrenergic receptors.
   Authors: Nakai A, Hayano Y, Furuta F, Noda M, Suzuki K
   J Exp Med, 2014;211(13):2583-98.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


9. Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress.
   Authors: Arnon, Tal I, Horton, Robert M, Grigorova, Irina L, Cyster, Jason G
   Nature, 2013;493(7434):684-8.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


10. Moesin controls clathrin-mediated S1PR1 internalization in T cells.
    Authors: Nomachi, Akira, Yoshinaga, Masanori, Liu, Jaron, Kanchanawong, Pakorn, Tohyama, Kiyoshi, Thumkeo, Dean, Watanabe, Takeshi, Narumiya, Shuh, Hirata, Takako
    PLoS ONE, 2013;8(12):e82590.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

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