抗Human ATG7抗体(Anti-Human ATG7 antibody)
掲載日情報：2018/11/26 現在Webページ番号：196419
Human ATG7に対する抗体(Anti-Human ATG7 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月04日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Human ATG7 MAb (Clone 683906) |
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Anti-Human/Mouse ATG7 MAb (Clone 683906) |
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[在庫・価格 ：2024年05月04日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human ATG7 MAb (Clone 683906)
文献数： 4
- 商品コード：MAB6608
- メーカー：RSD
- 包装：100μg
- 価格：¥100,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human/Mouse ATG7 MAb (Clone 683906)
文献数： 1
- 商品コード：MAB6608-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
Simple Western対応抗体。※受注発注品。形状：溶液または凍結乾燥 別名：APG7 クローン：683906 Genbank No： 10533 Protein Accession No： O95352 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | FCM,IC,IHC,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
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Product Details
| Species Reactivity | Human, Mouse |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human ATG7Asn387-Gln570Accession # O95352 |
| Source | Monoclonal Mouse IgG1 Clone # 683906 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human ATG7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human ATG3, 4A, 4B, 5, 10, 12, or 16L1 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 2 µg/mL | See below |
| Simple Western | 20 µg/mL | See below |
| Immunohistochemistry | 8-25 µg/mL | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Intracellular Staining by Flow Cytometry | 0.25 µg/106 cells | See below |
| Western Blot | |
|---|---|
 | Detection of Human ATG7 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ATG7 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunocytochemistry | |
 | ATG7 in RAW 264.7 Mouse Cell Line. ATG7 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Immunohistochemistry | |
 | ATG7 in Human Brain. ATG7 was detected in formalin fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
| Intracellular Staining by Flow Cytometry | |
 | Detection of ATG7 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
| Simple Western | |
 | Detection of Human ATG7 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATG7 at approximately 66 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
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Related Product & Information
| Long Name | ATG7 Autophagy Related 7 Homolog |
|---|---|
| Background | ATG7 |
| background_content | Background: ATG7 ATG7, also known as APG7, is a 78 kDa cytosolic ubiquitin-E1-like enzyme that plays a key role in the autophagic pathway of intracellular bulk degradation. It is required for the conjugation of ATG5 to ATG12, the lipidation of ATG8, and subsequent autophagosome formation. ATG7 is required for mitochondrial removal during erythropoiesis and for the maintenance of axonal homeostasis. Alternate splicing of human ATG7 generates an isoform that lacks 31 aa at the C-terminus, a region that is required for ATG8 lipidation. Human ATG7 shares 93% aa sequence identitiy with mouse and rat ATG7. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- Histone deacetylase 3 is required for iNKT cell development
Authors: P Thapa, S Romero Aro, JY Chung, DB Sant'Angel, VS Shapiro
Sci Rep, 2017;7(1):5784.
Species: Mouse
Sample Type: Whole Cells
Application: Flow - Altered mitochondrial quality control signaling in muscle of old gastric cancer patients with cachexia
Authors: Maurizio Bossola
Exp. Gerontol., 2016;0(0):.
Species: Human
Sample Type: Tissue Homogenates
Application: WB - Autophagy fosters myofibroblast differentiation through MTORC2 activation and downstream upregulation of CTGF.
Authors: Bernard M, Dieude M, Yang B, Hamelin K, Underwood K, Hebert M
Autophagy, 2014;10(12):2193-207.
Species: Human
Sample Type: Cell Lysates
Application: WB
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