抗Wnt-5a抗体(Anti-Wnt-5a, Rat-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	CHO-derived recombinant mouse Wnt-5aGln38-Lys380Accession # P22725
Source	Monoclonal Rat IgG2A Clone # 442625
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse Wnt-5a in direct ELISAs and human and mouse Wnt‑5a in Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Wnt-1, 2b, 3a, 4, 5b, 8a, 8b, 9b, 10a, 10b, 11, or 16 is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Immunohistochemistry	8-25 µg/mL	See below

Immunohistochemistry

Wnt‑5a in Mouse Embryo. Wnt‑5a was detected in immersion fixed frozen sections of mouse embryo using Human/Mouse Wnt‑5a Monoclonal Antibody (Catalog # MAB645) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti‑Rat IgG Secondary Antibody (orange; Catalog # NL013) and counter­stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

目次に戻る

Related Product & Information

Background

Wnt-5a

background_content

Background: Wnt-5a Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23‑24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development for a variety of organisms. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (1‑2). Wnt-5a is part of the subgroup of Wnts that are not axis-inducing in Xenopus embryos and do not transform C57MG mammary epithelial cells. This subgroup is also implicated in the Wnt/Ca2+ pathway, playing roles in cell movements and cell adhesion (3). This non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling. In Wnt-5a deficient mouse embryos, beta -catenin accumulates in the limb bud suggesting that Wnt-5a normally promotes degradation of beta -catenin (4). Likewise, in Xenopus embryos Wnt-5a antagonizes the ability of the canonical Wnt subgroup to induce a secondary axis (5). Wnt-5a is implicated in various types of cancer and has complex roles. It acts as a tumor suppressor for mammary, B-cell, colon, and uroepithelial cancer cells but is up-regulated in melanomas, where expression levels correlate with severity of metastasis (3). Furthermore, aberrant Wnt-5a signaling results in other diseases such as rheumatoid arthritis (6). Like other developmental growth factors Wnt-5a has diverse roles in development. They are too numerous to enunciate here, as functions span from early anterior-posterior development and gastrulation movements to maintaining hematopoietic stem cell population, lung morphogenesis, and limb outgrowth. Mature Wnt-5a is a 49 kDa protein that shares 99% amino acid identity in mouse, rat and human.

目次に戻る

Citations

1. ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling
   Authors: M Vesel, J Rapp, D Feller, E Kiss, L Jaromi, M Meggyes, G Miskei, B Duga, G Smuk, T Laszlo, I Karner, JE Pongracz
   Respir. Res, 2017;18(1):52.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Frozen


2. Distinct roles and differential expression levels of Wnt5a mRNA isoforms in colorectal cancer cells
   Authors: TC Huang, PT Lee, MH Wu, CC Huang, CY Ko, YC Lee, DY Lin, YW Cheng, KH Lee
   PLoS ONE, 2017;12(8):e0181034.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


3. WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition
   J Exp Clin Cancer Res, 2016;35(1):144.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD
   Authors: Hoeke A Baarsma
   J. Exp. Med, 2016;0(0):.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


5. Noncanonical Wnt signaling promotes obesity-induced adipose tissue inflammation and metabolic dysfunction independent of adipose tissue expansion.
   Authors: Fuster J, Zuriaga M, Ngo D, Farb M, Aprahamian T, Yamaguchi T, Gokce N, Walsh K
   Diabetes, 2015;64(4):1235-48.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


6. Oncogenic effects of WNT5A in Epstein-Barr virusassociated nasopharyngeal carcinoma.
   Authors: Yap L, Ahmad M, Zabidi M, Chu T, Chai S, Lee H, Lim P, Wei W, Dawson C, Teo S, Khoo A
   Int J Oncol, 2014;44(5):1774-80.
   Species: Human
   Sample Type: Protein
   Application: WB


7. Interleukin-6 drives melanoma cell motility through p38alpha-MAPK-dependent up-regulation of WNT5A expression.
   Authors: Linnskog R, Jonsson G, Axelsson L, Prasad C, Andersson T
   Mol Oncol, 2014;8(8):1365-78.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway.
   Authors: MacMillan, Connor D, Leong, Hon S, Dales, David W, Robertson, Amy E, Lewis, John D, Chambers, Ann F, Tuck, Alan B
   Sci Rep, 2014;4(0):6315.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


9. Wnt5a/beta-catenin signaling drives calcium-induced differentiation of human primary keratinocytes.
   Authors: Popp T, Steinritz D, Breit A, Deppe J, Egea V, Schmidt A, Gudermann T, Weber C, Ries C
   J Invest Dermatol, 2014;134(8):2183-91.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


10. Wnt5a promotes inflammatory responses via nuclear factor kappaB (NF-kappaB) and mitogen-activated protein kinase (MAPK) pathways in human dental pulp cells.
    Authors: Zhao Y, Wang C, Li R, Hui T, Su Y, Yuan Q, Zhou X, Ye L
    J Biol Chem, 2014;289(30):21028-39.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC

目次に戻る