抗UCP1抗体(Anti-UCP1, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant human UCP1Met1-Thr307Accession # P25874
Source	Monoclonal Mouse IgG2B Clone # 536435
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human and mouse UCP1 in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	2.5 µg/mL	See below
Immunocytochemistry	8-25 µg/mL	See below
Intracellular Staining by Flow Cytometry	0.25 µg/106 cells	See below

Western Blot
	Detection of Human UCP1 by Western Blot. Western blot shows recombinant human UCP1, recombinant human UCP2, recombinant human UCP3, and recombinant human UCP4 (5 ng/lane). PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western Blot
	Detection of Mouse UCP1 by Western Blot. Western blot shows lysates of mouse brown adipose tissue and mouse adipose tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UCP1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunocytochemistry
	UCP1 in Human Mesenchymal Stem Cells. UCP1 was detected in immersion fixed human mesenchymal stem cells undifferentiated (lower panel) or differentiated into adipocytes (upper panel) using Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Intracellular Staining by Flow Cytometry
	Detection of UCP1 in 3T3-L1 Mouse Cell Line by Flow Cytometry. 3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I(Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Simple Western
	Detection of Mouse UCP1 by Simple WesternTM. Simple Western lane view shows lysates of mouse adipose tissue and mouse brown adipose tissue, loaded at 0.5 mg/mL. A specific band was detected for UCP1 at approximately 37 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Uncoupling Protein 1
Background	UCP1
background_content	Background: UCP1 Mitochondrial brown fat uncoupling protein 1 (UCP1; also Thermogenin and UCP) is a 33 kDa member of the mitochondrial carrier family of proteins. Human and mouse UCP1 are both 307 amino acids (aa) in length and contain three solcar repetitive regions and six transmembrane segments. UCP1 is found in brown adipose tissue, where it becomes activated by fatty acids and inhibited by nucleotides. It functions as a mitochondrial transporter that creates a proton leak across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Human and mouse UCP1 share 79% aa sequence identity.

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Citations

1. Direct conversion of human fibroblasts to brown adipocytes by small chemical compounds
   Authors: Y Takeda, Y Harada, T Yoshikawa, P Dai
   Sci Rep, 2017;7(1):4304.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Both brown adipose tissue and skeletal muscle thermogenesis processes are activated during mild to severe cold adaptation in mice
   Authors: NC Bal, S Singh, FCG Reis, SK Maurya, S Pani, LA Rowland, M Periasamy
   J. Biol. Chem., 2017;0(0):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


3. IRX3 Promotes the Browning of White Adipocytes and Its Rare Variants are Associated with Human Obesity Risk
   Authors: Y Zou, P Lu, J Shi, W Liu, M Yang, S Zhao, N Chen, M Chen, Y Sun, A Gao, Q Chen, Z Zhang, Q Ma, T Ning, X Ying, J Jin, X Deng, B Shen, Y Zhang, B Yuan, S Kauderer, S Liu, J Hong, R Liu, G Ning, W Wang, W Gu, J Wang
   EBioMedicine, 2017;24(0):64-75.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-Fr


4. White-to-brown metabolic conversion of human adipocytes by JAK inhibition.
   Authors: Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C
   Nat Cell Biol, 2015;17(1):57-67.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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