抗Mouse CCR6抗体(Anti-Mouse CCR6 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	RBL-1 rat basophilic leukemia cell line transfected with mouse CCR6Met1-Met367Accession # O54689
Source	Monoclonal Rat IgG2A Clone # 140706
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse CCR6-transfected rat RBL cells but not the parent cell line or cells transfected with mouse CCR5.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
Immunohistochemistry	8-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry
	Detection of CCR6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with (A) Rat Anti-Mouse CCR6 Monoclonal Antibody (Catalog # MAB590) or (B) Rat IgG2A control antibody (Catalog # MAB006) followed by Goat anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113) and Rat Anti-Mouse B220/CD45R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1217F). View our protocol for Staining Membrane-associated Proteins.
Immunohistochemistry
	CCR6 in Mouse Thymus. CCR6 was detected in perfusion fixed frozen sections of mouse thymus using Mouse CCR6 Monoclonal Antibody (Catalog # MAB590) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
	CCR6 in Mouse Spleen. CCR6 was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse CCR6 Monoclonal Antibody (Catalog # MAB590) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Entrez Gene IDs	1235 (Human); 12458 (Mouse); 308163 (Rat)
Background	CCR6
background_content	Background: CCR6 CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

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Citations

1. IL-1? and IL-23 Promote Extrathymic Commitment of CD27(+)CD122(-) ?? T Cells to ??T17 Cells
   Authors: A Muschaweck, F Petermann, T Korn
   J. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require ?4?1 Integrin To Migrate between the Peritoneum and Inflamed Skin
   Authors: SA Geherin, D Gómez, RA Glabman, G Ruthel, A Hamann, GF Debes
   J. Immunol, 2016;196(6):2514-25.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. SerpinB1 regulates homeostatic expansion of IL-17+ gammadelta and CD4+ Th17 cells.
   Authors: Zhao P, Hou L, Farley K, Sundrud M, Remold-O'Donnell E
   J Leukoc Biol, 2014;95(3):521-30.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. CCR6 is a prognostic marker for overall survival in patients with colorectal cancer, and its overexpression enhances metastasis in vivo.
   Authors: Liu, Jinlin, Ke, Fang, Xu, Zhenyao, Liu, Zhaoyuan, Zhang, Lingyun, Yan, Sha, Wang, Zhe, Wang, Hong, Wang, Honglin
   PLoS ONE, 2014;9(6):e101137.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


5. CCR6 is required for epidermal trafficking of gammadelta-T cells in an IL-23-induced model of psoriasiform dermatitis.
   Authors: Mabuchi T, Singh T, Takekoshi T, Jia G, Wu X, Kao M, Weiss I, Farber J, Hwang S
   J Invest Dermatol, 2013;133(1):164-71.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


6. Colonic patch and colonic SILT development are independent and differentially regulated events.
   Authors: Baptista A, Olivier B, Goverse G, Greuter M, Knippenberg M, Kusser K, Domingues R, Veiga-Fernandes H, Luster A, Lugering A, Randall T, Cupedo T, Mebius R
   Mucosal Immunol, 2013;6(3):511-21.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IF


7. Programmed downregulation of CCR6 is important for establishment of epidermal gammadeltaT cells by regulating their thymic egress and epidermal location.
   Authors: Hu S, Xiong N
   J Immunol, 2013;190(7):3267-75.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


8. Human beta-defensin 2 and 3 and their mouse orthologs induce chemotaxis through interaction with CCR2.
   Authors: Rohrl J, Yang D, Oppenheim JJ, Hehlgans T
   J. Immunol., 2010;184(12):6688-94.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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