抗Human HNF-4 alpha/NR2A1抗体(Anti-Human HNF-4 alpha/NR2A1 antibody)
掲載日情報：2018/11/26 現在Webページ番号：196198
Human HNF-4 alpha/NR2A1に対する抗体(Anti-Human HNF-4 alpha/NR2A1 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月06日 18時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Human HNF-4 alpha/NR2A1 MAb (Clone 843716) |
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Anti-Human HNF-4 alpha/NR2A1 MAb (Clone 843716) |
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[在庫・価格 ：2024年05月06日 18時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human HNF-4 alpha/NR2A1 MAb (Clone 843716)
文献数： 0
- 商品コード：MAB4605
- メーカー：RSD
- 包装：100μg
- 価格：¥101,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
Simple Western対応抗体。 別名：FLJ39654 クローン：843716 Genbank No： 3172 Protein Accession No： P41235 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | FCM,IC,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
Mouse Anti-Human IL-17F Monoclonal Antibody / Mouse Anti-Human gp96/HSP90B1 Monoclonal Antibody |
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Anti-Human HNF-4 alpha/NR2A1 MAb (Clone 843716)
文献数： 0
- 商品コード：MAB4605-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
Simple Western対応抗体。※受注発注品。形状：溶液または凍結乾燥 別名：FLJ39654 クローン：843716 Genbank No： 3172 Protein Accession No： P41235 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | FCM,IC,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human HNF‑4 alpha /NR2A1Val130-Ser330Accession # P41235 |
| Source | Monoclonal Mouse IgG2B Clone # 843716 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human HNF‑4 alpha /NR2A1 in ELISAs and Western blots. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | See below |
| Simple Western | 10 µg/mL | See below |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Intracellular Staining by Flow Cytometry | 0.25 µg/106 cells | See below |
| Western Blot | |
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 | Detection of Human HNF‑4 alpha /NR2A1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. Gels were loaded with 10 µg of cytoplasmic (Cyto) and 5 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HNF‑4 alpha /NR2A1 Monoclonal Antibody (Catalog # MAB4605) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for HNF‑4 alpha /NR2A1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunocytochemistry | |
 | HNF-4 alpha /NR2A1 in HepG2 Human Cell Line. HNF‑4 alpha /NR2A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human HNF‑4 alpha /NR2A1 Monoclonal Antibody (Catalog # MAB4605) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Simple Western | |
 | Detection of Human HNF‑4 alpha /NR2A1 by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for HNF‑4 alpha /NR2A1 at approximately 62 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human HNF‑4 alpha /NR2A1 Monoclonal Antibody (Catalog # MAB4605). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. |
| Intracellular Staining by Flow Cytometry | |
 | Detection of HNF-4 alpha/NR2A1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human HNF-4 alpha/NR2A1 Monoclonal Antibody (Catalog # MAB4605, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules. |
| Intracellular Staining by Flow Cytometry | |
 | Detection of HNF-4 alpha/NR2A1 in Human Endodermal Cells by Flow Cytometry. BG01V human embryonic stem cell line differentiated to endodermal cells (StemXVivo Endoderm Kit, Catalog # SC019B) was stained with Mouse Anti-Human HNF-4 alpha/NR2A1 Monoclonal Antibody (Catalog # MAB4605, filled histogram or isotype control antibody (Catalog # MAB0041, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules. |
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Related Product & Information
| Long Name | Hepatocyte Nuclear Factor-4, alpha |
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| Background | HNF-4 alpha/NR2A1 |
| background_content | Background: HNF-4 alpha/NR2A1 HNF-4 alpha is a transcription factor that binds DNA as a homodimer. HNF‑4 alpha is important in liver, kidney, and intestinal development. It has also been intensely studied as one of a variety of genes responsible for diabetes mellitus. HNF-4 alpha has been shown in knock out mice to be essential for the morphogenic and functional differentiation of hepatocytes. HNF-4 alpha is a dominant regulator of epithelial phenotypes able to drive the mesenchymal-to-epithelial transition when expressed in fibroblasts. |
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