抗Human c-Myc抗体(Anti-Human c-Myc antibody)
掲載日情報：2018/11/26 現在Webページ番号：196124
Human c-Mycに対する抗体(Anti-Human c-Myc )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月02日 15時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Human c-Myc MAb (Clone 9E10) |
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[在庫・価格 ：2024年05月02日 15時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human c-Myc MAb (Clone 9E10)
文献数： 3
- 商品コード：MAB3696
- メーカー：RSD
- 包装：100μg
- 価格：¥99,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
別名：avian myelocytomatosis viral oncogene homolog クローン：9.00E+10 Genbank No： 4609 Protein Accession No： P01106 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | FCM,IC,IHC,IP,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
Anti-Tag Antibodies R&D Systems 社 抗c-Myc抗体 Fluorochrome-Conjugated Human Arginase 1 Monoclonal Antibodies |
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| 関連記事 | |||||||
Anti-Human c-Myc MAb (Clone 9E10)
文献数： 0
- 商品コード：MAB3696-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。形状：溶液または凍結乾燥 別名：avian myelocytomatosis viral oncogene homolog クローン：9.00E+10 Genbank No： 4609 Protein Accession No： P01106 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | FCM,IC,IHC,IP,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
Anti-Tag Antibodies |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | C-terminal region peptide of human c-MycAla408-Ala439Accession # P01106 |
| Source | Monoclonal Mouse IgG1 Clone # 9E10 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects endogenous human c-Myc and c-Myc tagged proteins in Western blots. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 2 µg/mL | See below |
| Flow Cytometry | 2.5 µg/106 cells | See below |
| Immunohistochemistry | 8-25 µg/mL | See below |
| Immunoprecipitation | Fan H, et al. (1998) Biochem. Cell. Biol. 76:125. | |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Knockout Validated | c‑Myc is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in c‑Myc knockout HEK293T cell line. | |
| Western Blot | |
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 | Detection of Human c‑Myc by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB3696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for c‑Myc at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Western Blot | |
 | Detection of c‑Myc-tagged Protein by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected (-) or transfected with c-Myc-tagged recombinant mouse Wnt-3a. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB3696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for c-Myc-tagged recombinant mouse Wnt-3a at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Flow Cytometry | |
 | Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB3696, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. |
| Immunocytochemistry | |
 | c-Myc in HEK293 Human Cell Line Transfected with c-Myc-tagged Serotonin Receptor. c‑Myc was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with c-Myc-tagged Serotonin Receptor using Mouse Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB3696) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Immunohistochemistry | |
 | c‑Myc in Human Prostate. c‑Myc was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB3696) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
| Knockout Validated | |
 | Western Blot Shows Human c‑Myc Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and c-Myc knockout HEK293T cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human c‑Myc Monoclonal Antibody (Catalog # MAB3696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for c‑Myc at approximately 52 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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Related Product & Information
| Long Name | v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian) |
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| Background | c-Myc |
| background_content | Background: c-Myc Human c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max. It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains. The 439 aa human c-Myc has one O-glycosylation site and has three Ser/Thr phosphorylation sites near the N-terminus. Human c-Myc shows 92% aa identity with mouse or rat c-Myc. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- U1 Adaptors suppress the KRAS-MYC oncogenic axis in human pancreatic cancer xenografts
Authors: AT Tsang, C Dudgeon, L Yi, X Yu, R Goraczniak, K Donohue, S Kogan, MA Brenneman, ES Ho, SI Gunderson, DR Carpizo
Mol. Cancer Ther., 2017;0(0):.
Species: Human
Sample Type: Cell Lysates
Application: WB - The Binding of the Bone Morphogenetic Protein Antagonist Gremlin to Kidney Heparan Sulfate: Such Binding is not Essential for BMP Antagonism
Authors: Arnold Junior Tatsinkam
Int. J. Biochem. Cell Biol, 2016;0(0):.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC-P
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