抗Mouse CD36/SR-B3抗体(Anti-Mouse CD36/SR-B3 antibody)

掲載日情報:2018/11/26 現在Webページ番号:196052

Mouse CD36/SR-B3に対する抗体(Anti-Mouse CD36/SR-B3 )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2025年04月26日 20時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Mouse CD36/SR-B3 MAb (Clone 324205)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 2
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説明文
別名:CD36
クローン:324205
Genbank No: 948
Protein Accession No: Q08857
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Rat クラス IgG 標識 Unlabeled
交差性 Mouse 適用 FCM,IHC
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
関連記事
Anti-Mouse CD36/SR-B3 MAb (Clone 324205)
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
  • メーカーサイト
  • お問い合わせ
説明文
※受注発注品。形状:溶液または凍結乾燥
別名:CD36
クローン:324205
Genbank No: 948
Protein Accession No: Q08857
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Rat クラス IgG 標識 Unlabeled
交差性 Mouse 適用 FCM,IHC
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
使いっきり抗体
関連記事

[在庫・価格 :2025年04月26日 20時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Mouse CD36/SR-B3 MAb (Clone 324205)

文献数: 2

説明文 別名:CD36
クローン:324205
Genbank No: 948
Protein Accession No: Q08857
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Rat
交差性 Mouse 適用 FCM,IHC
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
関連記事

Anti-Mouse CD36/SR-B3 MAb (Clone 324205)

文献数: 0

説明文 ※受注発注品。形状:溶液または凍結乾燥
別名:CD36
クローン:324205
Genbank No: 948
Protein Accession No: Q08857
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Rat
交差性 Mouse 適用 FCM,IHC
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
使いっきり抗体
関連記事



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Product Details

Species ReactivityMouse
LabelUnconjugated
ImmunogenChinese hamster ovary cell line CHO-derived recombinant mouse CD36/SR-B3Gly30-Lys439Accession # Q08857
SourceMonoclonal Rat IgG1 Clone # 324205
PurificationProtein A or G purified from hybridoma culture supernatant
SpecificityDetects mouse CD36/SR-B3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD36 is observed.


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Applications and Data

 Recommended
Concentration
Sample
Flow Cytometry2.5 µg/106 cellsSee below
Immunohistochemistry8-25 µg/mLSee below
CyTOF-readyReady to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.


Flow Cytometry
Detection of CD36/SR‑B3 in J774A.1 Mouse Cell Line by Flow Cytometry.
J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse CD36/SR‑B3 Monoclonal Antibody (Catalog # MAB25191, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Immunohistochemistry
CD36/SR‑B3 in Mouse Heart.
CD36/SR‑B3 was detected in immersion fixed frozen sections of mouse heart using Rat Anti-Mouse CD36/SR‑B3 Monoclonal Antibody (Catalog # MAB25191) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
CD36/SR‑B3 in Mouse Thymus.
CD36/SR‑B3 was detected in immersion fixed frozen sections of mouse thymus using Rat Anti-Mouse CD36/SR‑B3 Monoclonal Antibody (Catalog # MAB25191) at 15 µg/mL overnight at4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.


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Related Product & Information

BackgroundCD36/SR-B3
background_contentBackground:
CD36/SR-B3
CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N‑terminal cytoplasmic domain, a 22 aa N‑terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C‑terminal transmembrane segment, and a 9 aa C‑terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C‑terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N‑terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 shares 94%, 92%, 84% and 84% aa sequence identity with the extracellular loops of rat, hamster, human and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells and hematopoietic cells such as RBC’s, platelets and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. CD36 deletion reduces VLDL secretion, modulates liver prostaglandins, and exacerbates hepatic steatosis in ob/ob mice.
    Authors: Nassir F, Adewole O, Brunt E, Abumrad N
    J Lipid Res, 2013;54(11):2988-97.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC

  2. Hematopoietic cell-restricted deletion of CD36 reduces high-fat diet-induced macrophage infiltration and improves insulin signaling in adipose tissue.
    Authors: Nicholls HT, Kowalski G, Kennedy DJ
    Diabetes, 2011;60(4):1100-10.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB



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