抗Human CXCR5抗体(Anti-Human CXCR5 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	NS0 mouse myeloma cell line transfected with human CXCR5Met1-Phe372Accession # P32302
Source	Monoclonal Mouse IgG2B Clone # 51505
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
Immunohistochemistry	8-25 µg/mL	See below
CyTOF-reported	Ferrell, P.B., Jr. et al. (2016) PLoS ONE 11: e0153207. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
Neutralization	Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 0.25-1.5 µg/mL in the presence of 0.05 µg/mL Recombinant Human CXCL13/BLC/BCA‑1.

Flow Cytometry
	Detection of CXCR5 in CD19+ Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) or (B) Mouse IgG2B control antibody (Catalog # MAB0041) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
	CXCR5 in Human PBMCs. CXCR5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 637-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL008) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
	CXCR5 in Human Kidney. CXCR5 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization
	Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCR5 Antibody. Recombinant Human CXCL13/BLC/BCA‑1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose‑dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL13/BLC/BCA‑1 (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190). The ND50 is typically0.25-1.5 µg/mL.

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Related Product & Information

Entrez Gene IDs	643 (Human); 12145 (Mouse); 29363 (Rat)
Background	CXCR5
background_content	Background: CXCR5 CXCR5, also known as BLR-1, is a 7 transmembrane domain protein expressed on B cells. CXCR5 mediates B cell migration following binding of CXCL13.

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Citations

1. p53-dependent expression of CXCR5 chemokine receptor in MCF-7 breast cancer cells.
   Authors: Mitkin N, Hook C, Schwartz A, Biswas S, Kochetkov D, Muratova A, Afanasyeva M, Kravchenko J, Bhattacharyya A, Kuprash D
   Sci Rep, 2015;5(0):9330.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Capture-stabilize approach for membrane protein SPR assays.
   Authors: Chu, Ruiyin, Reczek, David, Brondyk, William
   Sci Rep, 2014;4(0):7360.
   Species: Human
   Sample Type: Peptide
   Application: Biacore


3. The MEC1 and MEC2 lines represent two CLL subclones in different stages of progression towards prolymphocytic leukemia.
   Authors: Rasul E, Salamon D, Nagy N, Leveau B, Banati F, Szenthe K, Koroknai A, Minarovits J, Klein G, Klein E
   PLoS ONE, 2014;9(8):e106008.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. CXCR7 influences the migration of B cells during maturation.
   Authors: Humpert M, Pinto D, Jarrossay D, Thelen M
   Eur J Immunol, 2014;44(3):694-705.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency.
   Authors: Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A
   J Allergy Clin Immunol, 2013;131(4):1146-56, 1156.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. IL-15 positively regulates IL-21 production in celiac disease mucosa.
   Authors: Sarra M, Cupi M, Monteleone I, Franze E, Ronchetti G, Di Sabatino A, Gentileschi P, Franceschilli L, Sileri P, Sica G, Del Vecchio Blanco G, Cretella M, Paoluzi O, Corazza G, Pallone F, Monteleone G
   Mucosal Immunol, 2013;6(2):244-55.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Frozen


7. Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells.
   Authors: Islam SA, Chang DS, Colvin RA
   Nat. Immunol., 2011;12(2):167-77.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion.
   Authors: Singh S, Singh R, Sharma PK, Singh UP, Rai SN, Chung LW, Cooper CR, Novakovic KR, Grizzle WE, Lillard JW
   Cancer Lett., 2009;283(1):29-35.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection.
   Authors: Cagigi A, Mowafi F, Phuong Dang LV, Tenner-Racz K, Atlas A, Grutzmeier S, Racz P, Chiodi F, Nilsson A
   Blood, 2008;112(12):4401-10.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


10. Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients.
    Authors: Panse J, Friedrichs K, Marx A, Hildebrandt Y, Luetkens T, Bartels K, Horn C, Stahl T, Cao Y, Milde-Langosch K, Niendorf A, Kroger N, Wenzel S, Leuwer R, Bokemeyer C, Hegewisch-Becker S, Atanackovic D
    Br. J. Cancer, 2008;99(6):930-8.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB

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