抗Human B7-H1抗体(Anti-Human B7-H1 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human PD-L1/B7-H1Phe19-Thr239Accession # Q9NZQ7
Source	Monoclonal Mouse IgG1 Clone # 130021
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human PD-L1/B7-H1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-1, -2, -H2, -H3, -H3b,-H4, rhPD-L2, recombinant mouse B7-H1, recombinant rat (rr) B7-1, or rrB7-2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
Immunohistochemistry	8-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry
	Detection of PD-L1/B7-H1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Immunohistochemistry
	PD-L1/B7-H1 in Human Colon Cancer. PD-L1/B7-H1 was detected in formalin fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was observed in the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Background

PD-L1/B7-H1

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Background: PD-L1/B7-H1 Human B7 homolog 1 (B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. Other family members include B7-1, B7-2, B7-H2, PDL2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily. Their extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, there is about 20-25% amino acid identity. Human and mouse B7-H1 share approximately 70% amino acid sequence identity. B7-H1 has been identified as one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The B7-H1 gene encodes a 291 amino acid (aa) type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular domain, a 21 aa transmembrane region, and a 31 aa cytoplasmic domain. Human B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN-gamma stimulation. Interaction of B7-H1 with PD-1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.

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Citations

1. Soluble PD-L1 as a biomarker in malignant melanoma and checkpoint blockade
   Authors: J Zhou, KM Mahoney, A Giobbie-Hu, F Zhao, S Lee, X Liao, S Rodig, J Li, X Wu, LH Butterfiel, M Piesche, MP Manos, LM Eastman, G Dranoff, GJ Freeman, FS Hodi
   Cancer Immunol Res, 2017;0(0):.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Capture


2. Hilar fat infiltration: A new prognostic factor in metastatic clear cell renal cell carcinoma with first-line sunitinib treatment
   Authors: SF Kammerer-J, A Brunot, K Bensalah, B Campillo-G, M Lefort, S Bayat, A Ravaud, F Dupuis, M Yacoub, G Verhoest, B Peyronnet, R Mathieu, A Lespagnol, J Mosser, J Edeline, B Laguerre, JC Bernhard, N Rioux-Lecl
   Urol. Oncol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC-P


3. Overexpression of B7-H1 correlates with malignant cell proliferation in pancreatic cancer.
   Authors: Song, Xiao, Liu, Junwei, Lu, Yi, Jin, Hongchua, Huang, Dongshen
   Oncol Rep, 2014;31(3):1191-8.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


4. IFN-gamma generates maturation-arrested dendritic cells that induce T cell hyporesponsiveness independent of Foxp3+ T-regulatory cell generation.
   Authors: Rojas D, Krishnan R,
   Immunol. Lett., 2010;132(1):31-7.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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