抗Mouse CCL3/MIP-1 alpha Alexa Fluor 488抗体(Anti-Mouse CCL3/MIP-1 alpha Alexa Fluor 488 antibody)

掲載日情報:2018/11/26 現在Webページ番号:195880

Mouse CCL3/MIP-1 alpha Alexa Fluor 488に対する抗体(Anti-Mouse CCL3/MIP-1 alpha Alexa Fluor 488 )です。
本製品は研究用です。研究用以外には使用できません。

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Anti-Mouse CCL3/MIP-1 alpha Alexa Fluor 488 MAb (Clone 39624)
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説明文
別名:C-C motif chemokine 3
クローン:39624
Genbank No: 6348
Protein Accession No: P10855
法規制等
保存条件 4℃,暗所保存,凍結禁止 法規備考
抗原種 免疫動物 Rat クラス IgG 標識 Alexa Fluor 488
交差性 Mouse 適用 FCM
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
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製品記事 M1/M2 Macrophage Activation Marker
関連記事

[在庫・価格 :2025年04月26日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Mouse CCL3/MIP-1 alpha Alexa Fluor 488 MAb (Clone 39624)

文献数: 0

説明文 別名:C-C motif chemokine 3
クローン:39624
Genbank No: 6348
Protein Accession No: P10855
法規制等
保存条件 4℃,暗所保存,凍結禁止 法規備考
抗原種 免疫動物 Rat
交差性 Mouse 適用 FCM
標識 Alexa Fluor 488 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
関連記事



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Product Details

Species ReactivityMouse
LabelAlexa Fluor 488
ImmunogenE. coli-derived recombinant mouse CCL3/MIP-1 alpha Ala24-Ala92Accession # P10855
SourceMonoclonal Rat IgG2A Clone # 39624
PurificationProtein A or G purified from hybridoma culture supernatant
SpecificityDetects mouse CCL3/MIP‑1 alpha in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse CCL4/MIP‑1 beta  is observed and no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 21, 22, 25, 28, recombinant rat CCL2 or CCL20 is observed. Neutralizes the biological activity of recombinant mouse CCL3 and will not block the biological activity of recombinant human CCL3.


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Applications and Data

 Recommended
Concentration
Sample
Intracellular Staining by Flow Cytometry5 µL/106 cellsSee below


Intracellular Staining by Flow Cytometry
Detection of CCL3/MIP‑1 alpha in RAW 264.7 Mouse Cell Line by Flow Cytometry.
RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCL3/MIP‑1 alpha Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC450G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.


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Related Product & Information

BackgroundCCL3/MIP-1 alpha
background_contentBackground:
CCL3/MIP-1 alpha
The macrophage inflammatory proteins 1 alpha and 1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse CCL3/MIP-1 alpha shares approximately 77% and 70% amino acid identity with human CCL3/MIP-1 alpha and mouse CCL4/MIP-1 beta, respectively. MIP‑1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP‑1 proteins are members of the beta  (C‑C) subfamily of chemokines. Both CCL3 and CCL4 are monocyte chemoattractants in vitro. Additionally, the MIP‑1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with CCL3 and CCL4 preferentially attracting CD8+ and CD4+ T cells, respectively. CCL3 has also been shown to attract B cells as well as eosinophils. MIP‑1 proteins have been reported to have multiple effects on hematopoietic precursor cells, and CCL3 has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, CCL4 was reported to be much less active. The functional receptor for CCL3 has been identified as CCR1 and CCR5.


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