抗Human CCL4/MIP-1 beta Phycoerythrin抗体(Anti-Human CCL4/MIP-1 beta Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta Ala24-Asn92Accession # P13236
Source	Monoclonal Mouse IgG2B Clone # 24006
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In Western blots, this antibody shows less than 4% cross-reactivity with recombinant human (rh) CCL3/MIP‑1 alpha and does not cross-react with recombinant mouse (rm) CCL3/MIP‑1 alpha, rmCCL4/MIP‑1 beta, rhCXCL8/IL‑8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of CCL4/MIP‑1 beta in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS and Monensin for 5 hours were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCL4/MIP‑1 beta PE‑conjugated Monoclonal Antibody (Catalog # IC271P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Entrez Gene IDs	6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine)
Background	CCL4/MIP-1 beta
background_content	Background: CCL4/MIP-1 beta CCL4, also known as Macrophage Inflammatory Protein 1 beta (MIP-1 beta ) is a member of the CC or beta chemokine subfamily. CCL4 is expressed primarily by T cells, B cells, and monocytes after antigen or mitogen stimulation. The functional receptor for CCL4 has been identified as CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.

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Citations

1. DNA Methylation Regulates the Differential Expression of CX3CR1 on Human IL-7Ralphalow and IL-7Ralphahigh Effector Memory CD8+ T Cells with Distinct Migratory Capacities to the Fractalkine.
   Authors: Shin M, You S, Kang Y, Lee N, Yoo S, Park K, Kang K, Kim S, Mohanty S, Shaw A, Montgomery R, Hwang D, Kang I
   J Immunol, 2015;195(6):2861-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Increased memory differentiation is associated with decreased polyfunctionality for HIV but not for cytomegalovirus-specific CD8+ T cells.
   Authors: Riou C, Treurnicht F, Abrahams M, Mlisana K, Liu M, Goonetilleke N, Koup R, Roederer M, Abdool Karim S, de Bruyn G, Williamson C, Gray C, Burgers W
   J Immunol, 2012;189(8):3838-47.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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