抗SOX17抗体(Anti-SOX17, Goat-Poly, APC antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Allophycocyanin
Immunogen	E. coli-derived recombinant human SOX17Asp177-Val414Accession # Q9H6I2
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human SOX17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX2, rhSOX6, and rhSOX15 is observed.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of SOX17 in Definitive Endoderm-differentiated BG01V Human Stem Cells by Flow Cytometry. Definitive endoderm-differentiated (Catalog # SC019) BG01V human embryonic stem cells were stained with Goat Anti-Human SOX17 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1924A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Background	SOX17
background_content	Background: SOX17 SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2). In Western blot, AF1924 detects a 60 kDa band of endogenous SOX17 which is higher than predicted molecular weight but consistent with the current literature (3).

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Citations

1. Single-cell RNA sequencing reveals metallothionein heterogeneity during hESC differentiation to definitive endoderm
   Authors: J Lu, A Baccei, E Lummertz d, C Guillermie, S McManus, LA Finney, C Zhang, ML Steinhause, H Li, PH Lerou
   Stem Cell Res, 2018;28(0):48-55.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. DMSO efficiently down regulates pluripotency genes in human embryonic stem cells during definitive endoderm derivation and increases the proficiency of hepatic differentiation.
   Authors: Czysz, Katherin, Minger, Stephen, Thomas, Nick
   PLoS ONE, 2015;10(2):e0117689.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells.
   Authors: Wong, Amy P, Chin, Stephani, Xia, Sunny, Garner, Jodi, Bear, Christin, Rossant, Janet
   Nat Protoc, 2015;10(3):363-81.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture.
   Authors: Takeuchi, Hiroki, Nakatsuji, Norio, Suemori, Hirofumi
   Sci Rep, 2014;4(0):4488.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. BRACHYURY and CDX2 mediate BMP-induced differentiation of human and mouse pluripotent stem cells into embryonic and extraembryonic lineages.
   Authors: Bernardo AS, Faial T, Gardner L, Niakan KK, Ortmann D, Senner CE, Callery EM, Trotter MW, Hemberger M, Smith JC, Bardwell L, Moffett A, Pedersen RA
   Cell Stem Cell, 2010;9(2):144-55.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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