抗Human Galectin-3 Alexa Fluor 488抗体(Anti-Human Galectin-3 Alexa Fluor 488 antibody)
掲載日情報：2018/11/26 現在Webページ番号：195793
Human Galectin-3 Alexa Fluor 488に対する抗体(Anti-Human Galectin-3 Alexa Fluor 488 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月25日 08時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
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文献数 | ||
|---|---|---|---|---|---|
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Anti-Human Galectin-3 Alexa Fluor 488 Affinity Purified PAb (100 TESTS) |
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1 | |||
[在庫・価格 ：2024年05月25日 08時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human Galectin-3 Alexa Fluor 488 Affinity Purified PAb (100 TESTS)
文献数： 1
- 商品コード：IC1154G
- メーカー：RSD
- 包装：100tests
- 価格：¥86,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Alexa Fluor 488 |
| Immunogen | E. coli-derived recombinant human Galectin‑3Ala2-Ile250Accession # P17931.5 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human Galectin-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse Galectin-3 is observed and less than 1% cross-reactivity with recombinant human Galectin-1, -2, -4, -7, and -8 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Intracellular Staining by Flow Cytometry | 5 µL/106 cells | See below |
| Intracellular Staining by Flow Cytometry | |
|---|---|
 | Detection of Galectin‑3 in PBMC monocytes by Flow Cytometry. Peripheral blood mononuclear cell (PBMC) monocytes were stained with Goat Anti-Human Galectin‑3 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1154G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
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Related Product & Information
| Entrez Gene IDs | 3958 (Human); 16854 (Mouse) |
|---|---|
| Background | Galectin-3 |
| background_content | Background: Galectin-3 The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins. Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is a chimera galectin that has a tendency to dimerize. Besides the soluble protein, alternatively spliced forms of chicken Galectin-3 containing a transmembrane-spanning domain and a leucine zipper motif have been reported. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells, and fibroblasts. It binds various matrix glycoproteins including laminin, fibronectin, LAMPS, 90K/Mac-2BP, MP20, and CEA. Galectin-3 promotes cell growth and proliferation for many cell types. Galectin-3 acts intracellularly to prevent apoptosis. Depending on the cell types, Galectin-3 exhibits pro- or anti-adhesive properties. Galectin-3 has pro-inflammatory activities in vitro and in vivo. It induces pro-inflammatory and inhibits Th2 type cytokine production. Galectin-3 chemoattracts monocytes and macrophages. It activates and degranulates basophils and mast cells. Elevated circulating levels of Galectin-3 has been show to correlate with the malignant potential of several types of cancer, suggesting that Galectin-3 is also involved in tumor growth and metastasis. Human and mouse Galectin-3 shares approximately 80% amino acid sequence identity. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- Notochordal and nucleus pulposus marker expression is maintained by sub-populations of adult human nucleus pulposus cells through aging and degeneration
Authors: SM Richardson, FE Ludwinski, KK Gnanalingh, RA Atkinson, AJ Freemont, JA Hoyland
Sci Rep, 2017;7(1):1501.
Species: Human
Sample Type: Tissue Homogenates
Application: Flow
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