抗Mouse ICAM-1/CD54 Phycoerythrin抗体(Anti-Mouse ICAM-1/CD54 Phycoerythrin antibody)

掲載日情報:2018/11/26 現在Webページ番号:195747

Mouse ICAM-1/CD54 Phycoerythrinに対する抗体(Anti-Mouse ICAM-1/CD54 Phycoerythrin )です。
本製品は研究用です。研究用以外には使用できません。

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納期 文献数
Anti-Mouse ICAM-1/CD54 Phycoerythrin MAb (Clone 166623)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 1
説明文
別名:BB2
クローン:166623
Genbank No: 3383
Protein Accession No: P13597
法規制等
保存条件 4℃,暗所保存,凍結禁止 法規備考
抗原種 免疫動物 Rat クラス IgG 標識 PE
交差性 Mouse 適用 FCM
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
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[在庫・価格 :2024年04月26日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Mouse ICAM-1/CD54 Phycoerythrin MAb (Clone 166623)

文献数: 1

説明文 別名:BB2
クローン:166623
Genbank No: 3383
Protein Accession No: P13597
法規制等
保存条件 4℃,暗所保存,凍結禁止 法規備考
抗原種 免疫動物 Rat
交差性 Mouse 適用 FCM
標識 PE 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事
関連記事



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Product Details

Species ReactivityMouse
LabelPhycoerythrin
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse ICAM-1Gln28-Asn485Accession # P13597
SourceMonoclonal Rat IgG2B Clone # 166623
PurificationProtein A or G purified from hybridoma culture supernatant
SpecificityDetects mouse ICAM-1 in ELISAs and Western blots. In Western blots, this antibody shows weak cross-reactivity with recombinant human (rh) ICAM‑1 and recombinant mouse (rm) ICAM-2 and no cross-reactivity with rhCD31, rmDCC, rhICAM-2, rhICAM-3, recombinant rat (rr) ICAM-1, rmMAdCAM-1, or rmVCAM-1.


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Applications and Data

 Recommended
Concentration
Sample
Flow Cytometry10 µL/106 cellsSee below


Flow Cytometry
Detection of ICAM‑1/CD54 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse ICAM‑1/CD54 PE‑conjugated Monoclonal Antibody (Catalog # FAB796P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.


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Related Product & Information

Long NameIntercellular Adhesion Molecule 1
BackgroundICAM-1/CD54
background_contentBackground:
ICAM-1/CD54
ICAM-1 (InterCellular Adhesion Molecule-1), also known as CD54, is an 85-93 kDa member of the Immunoglobulin supergene family of molecules. Mouse ICAM-1 is a 510 amino acid (aa) type I transmembrane glycoprotein that contains five C2-type Ig-like domains in a 458 aa extracellular domain (ECD) (aa 28-485). It is expressed constitutively by, or inducibly on, a very wide variety of cells, including neurons, dendritic cells, fibroblasts, skeletal muscle myocytes, keratinocytes, eosinophils, neutrophils, hepatocytes, monocytes and vascular endothelial cells. On the cell surface, it exists primarily as an "intermediate" between a homodimeric and monomeric state. The homodimeric pairing does not seem to offer a distinct advantage with respect to ligand binding. On the other hand, the degree of glycosylation has a marked impact on ICAM-1 ligand binding, with under-glycosylation associated with low affinity ligand binding. Ligands for ICAM-1 include LFA-1 ( alpha L beta 2), Mac-1 ( alpha M beta 2), CD11c/CD18, fibrinogen, hyaluronan and select rhinoviruses. Notably, ICAM-1 is recognized to be a potential coreceptor for HGF, acting in concert with c-Met during ligand binding. ICAM-1 is best known for its role in white cell trafficking (leukocyte binding to endothelium), and is also reported to play a key role in the establishment of CD8+ T cell memory, serving as a link between mature dendritic cells and naïve CD8+ T cells. Over its ECD, mouse ICAM-1 shares 78% and 54% aa sequence identity with rat and human ICAM-1 ECD, respectively. There is one isoform variant for mouse ICAM-1 listed in SwissProt that shows a 28 aa substitution for aa 1-34.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. ICAM-1-based rabies virus vaccine shows increased infection and activation of primary murine B cells in vitro and enhanced antibody titers in-vivo.
    Authors: Norton, James E, Lytle, Andrew G, Shen, Shixue, Tzvetkov, Evgeni P, Dorfmeier, Corin L, McGettigan, James P
    PLoS ONE, 2014;9(1):e87098.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow



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