抗Human Connexin 43/GJA1 Allophycocyanin抗体(Anti-Human Connexin 43/GJA1 Allophycocyanin antibody)

掲載日情報:2018/11/26 現在Webページ番号:195738

Human Connexin 43/GJA1 Allophycocyaninに対する抗体(Anti-Human Connexin 43/GJA1 Allophycocyanin )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2024年05月18日 09時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-Human Connexin 43/GJA1 Allophycocyanin MAb (Clone 578618)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 3
説明文
別名:connexin 43
クローン:578618
Genbank No: 2697
Protein Accession No: P17302
法規制等
保存条件 4℃,暗所保存,凍結禁止 法規備考
抗原種 免疫動物 Mouse クラス IgG 標識 APC
交差性 Human 適用 FCM
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
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[在庫・価格 :2024年05月18日 09時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Human Connexin 43/GJA1 Allophycocyanin MAb (Clone 578618)

文献数: 3

説明文 別名:connexin 43
クローン:578618
Genbank No: 2697
Protein Accession No: P17302
法規制等
保存条件 4℃,暗所保存,凍結禁止 法規備考
抗原種 免疫動物 Mouse
交差性 Human 適用 FCM
標識 APC 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事
関連記事



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Product Details

Species ReactivityHuman
LabelAllophycocyanin
ImmunogenMouse myeloma cell line NS0 transfected with human Connexin 43/GJA1Accession # P17302
SourceMonoclonal Mouse IgG2A Clone # 578618
PurificationProtein A or G purified from hybridoma culture supernatant
SpecificityDetects human Connexin 43/GJA1 in direct ELISAs.


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Applications and Data

 Recommended
Concentration
Sample
Flow Cytometry10 µL/106 cellsSee below


Flow Cytometry
Detection of Connexin 43/GJA1 in HEK293 Human Cell Line Transfected with Human Connexin 43/GJA1 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human Connexin 43/GJA1 and eGFP was stained with and either (A) Mouse Anti-Human Connexin 43/GJA1 APC‑conjugated Monoclonal Antibody (Catalog # FAB7737A) or (B) Mouse IgG2A Allophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.


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Related Product & Information

BackgroundConnexin 43/GJA1
background_contentBackground:
Connexin 43/GJA1
Connexin43 (Cx43; also gap junction alpha -1 protein) is a 41 - 44 kDa member of the connexin family, alpha -type subfamily, of transmembrane proteins. It is the most common type of connexin in cardiac muscle cells, and also occurs in hepatocytes, astrocytes and ovary granulosa cells. It is a 4-transmembrane protein 382 amino acids (aa) in length that contains two cytoplasmic tails. One is 12 aa in length at the N-terminus and the second is 151 aa in length at the C-terminus. Human and rat Cx43 are 98% aa identical over the entire length of the molecule. Connexins form gap junctions (GJs) which are intercellular channels between cells. Each adjacent cell contributes to a functional channel. The fundamental unit is a “connexon”, or hemi-channel, which is composed of six connexins in a sliding subunit hexamer configuration. The hexamer may be either homomeric or heteromeric. The connexon arrangement provides for the opening and closing of an intersubunit space (or pore) that allows diffusion of molecules 1 kDa or less. It is suggested that the extended C-terminus of each connexin may interact with multiple docking proteins and serve as a plug during closure. Connexons on adjacent cells interact via their extracellular loops to form a GJ channel. When open, these channels allow for the transit of small hydrophilic molecules such as ATP, glucose, IP3 and Ca++. Connexons (or hemi-channels) that do not interact with adjacent cells are also suggested to allow for small molecule transit under unusual circumstances. Cx43 activity is regulated by phosphorylation. The exact effects, however, may be context-specific. In one case, PKC phosphorylation of S368 has been found to decrease GJ channel permeability to select small molecular weight solutes. Alternatively, PKA has been suggested to be the mediator of Cx43 phosphorylation of S365, S368, S369 and S373. In this case, phosphorylation promotes channel activity.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells
    Authors: P Szaraz, M Librach, L Maghen, F Iqbal, TA Barretto, S Kenigsberg, A Gauthier-F, CL Librach
    Stem Cells Int, 2016;2016(0):7513252.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow



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