抗Mouse/Rat DARC Allophycocyanin抗体(Anti-Mouse/Rat DARC Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

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Product Details

Species Reactivity	Mouse, Rat
Label	Allophycocyanin
Immunogen	E. coli-derived recombinant mouse DARCMet1-Pro61, Ala115-Cys127, Ser186-Lys205, Tyr264-Asn285Accession # NP_034175
Source	Polyclonal Sheep IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse and rat DARC in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of DARC in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse TER‑119 PE‑conjugated Monoclonal Antibody (Catalog # FAB1125P) and either (A) Sheep Anti-Mouse/Rat DARC APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6695A) or (B) Normal Sheep IgG Allophycocyanin Control (Catalog # IC016A). View our protocol for Staining Membrane-associated Proteins.
Flow Cytometry
	Detection of DARC in HEK293 Human Cell Line Transfected with Mouse DARC and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse DARC and eGFP was stained with either (A) Sheep Anti-Mouse/Rat DARC APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6695A) or (B) Normal Sheep IgG Allophycocyanin Control (Catalog # IC016A). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Long Name	Duffy Antigen, Receptor For Chemokines
Background	DARC
background_content	Background: DARC DARC (Duffy Antigen Receptor for Chemokines; also CD234) is a 40-46 kDa glycoprotein member of the Duffy family of silent heptahelical chemokine receptors. It is expressed in liver and on select neurons, erythrocytes and the endothelium of postcapillary venules. Unlike traditional chemokine receptors, DARC cannot signal through G-proteins as it lacks a DRYLAIVHA cytoplasmic motif. DARC has three potential functions: first, it binds circulating inflammatory-type chemokines, serving as a repository for future chemokine release; second, it acts as a vehicle by which chemokines are transported from the abluminal to the luminal side of endothelium; and third, it complexes with signal-transducing chemokine receptors to create a nonsignaling heterodimer. Mouse DARC is 334 amino acids (aa) in length. It contains a 62 aa N-terminal extracellular region, and a 28 aa C-terminal cytoplasmic tail. There is one potential splice variant that shows a 42 aa substitution for aa 133-334. Collectively, over the four extracellular domains (aa 1-62, 115-127, 186-205, 264-285), mouse DARC shares 52% and 75% aa identity with human and rat DARC, respectively.

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