抗Human MSP R/Ron PerCP抗体(Anti-Human MSP R/Ron PerCP antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

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Product Details

Species Reactivity	Human
Label	PerCP (Peridinin-chlorophyll Protein Complex)
Immunogen	Mouse myeloma cell line NS0-derived recombinant human MSP R/RonGlu25-Ser956Accession # Q04912
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Delects human MSP R/Ron in direct ELISAs and Western blots. In these formats, approximately 25% cross‑reactivity with recombinant mouse MSP R is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of MSP R/Ron in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Goat Anti-Human MSP R/Ron PerCP‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6647C, filled histogram) or isotype control antibody (Catalog # IC108C, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Long Name	Macrophage Stimulating Protein Receptor
Background	MSP R/Ron
background_content	Background: MSP R/Ron RON (Recepteur d'Origine Nantais [Receptor originating (or discovered) in Nantes (France)]; also known as MSP-R, PTK8 and MST1R) is a 180-190 kDa member of the tyrosine kinase family, protein kinase superfamily of molecules. Human mature RON is a type I transmembrane heterodimeric glycoprotein that arises from the proteolytic cleavage of a single chain proform 1376 amino acids (aa) in length. Posttranslational processing generates a 35-40 kDa a-chain (aa 25-304) coupled to a 145-150 kDa b-chain (aa 310-1400). The chains are disulfide-linked, and the transmembrane segment appears over aa 958-978. Multiple cell types are known to express RON, and these include osteoclasts, TrkA+ dorsal root ganglia neurons, macrophages, keratinized and nonkeratinized stratified squamous epithelium, intestinal Paneth cells, renal proximal tubule and mesangial cells, monocytes, neutrophils and mammary epithelium. On the cell surface, RON serves as a receptor for MSP, a 78-80 kDa soluble molecule related to HGF. Ligand binding initiates homodimerization and receptor signaling. In addition, RON is also known to heterodimerize with multiple integral membrane proteins, either individually or as part of a complex. Proteins identified to date include b1 integrins, c-MET/HGFR, E-Cadherin, the bc-chain for IL-3, EPO-R, EGFR, IGF-IR, Plexins B1 and B3, and CD44v6. RON may also be found intracellularly, where it interacts with either the androgen receptor or EGFR in the cytoplasm, or in the nucleus, where it acts as a transcription factor coupled to HIF-1a and regulates c-Jun, Bcl-2 and c-FLIP expression. There are about a dozen alternative splice forms for RON, some soluble, while others are transmembrane, and either constitutively active or biochemically inert. They hold the potential to interact with full-length RON and impact its signaling capabilities. Reflecting the diverse nature of RON-expressing cells, RON has been found to generate multiple outcomes, including the promotion of epithelial-to-mesenchymal transitions, the maintenance of M2 macrophage phenotypes, and the initiation of keratinocyte proliferation and migration. Over aa 25-956, human and mouse RON share 76% aa sequence identity.

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