抗IL-1 RⅡ抗体(Anti-IL-1 RⅡ, Mouse-Mono, PE antibody)

掲載日情報:2018/11/26 現在Webページ番号:195677

IL-1 RⅡに対する抗体(Anti-IL-1 RⅡ, Mouse-Mono, PE )です。
本製品は研究用です。研究用以外には使用できません。

価格

[在庫・価格 :2024年04月30日 16時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
詳細 商品名
  • 商品コード
  • メーカー
  • 包装
  • 価格
  • 在庫
  • 法規制等
納期 文献数
Anti-IL-1 RII, Mouse-Mono(34141), PE <Anti-IL-1 Receptor II>
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 2
説明文
別名:beta
クローン:34141
Genbank No: 7850
Protein Accession No: P27930
法規制等
保存条件 4℃,暗所保存,凍結禁止 法規備考
抗原種 免疫動物 Mouse クラス IgG 標識 PE
交差性 Human 適用 FCM
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 MagCellect™ Mouse and Human Natural Killer Cell Isolation Kits
M1/M2 Macrophage Activation Marker
関連記事

[在庫・価格 :2024年04月30日 16時35分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-IL-1 RII, Mouse-Mono(34141), PE <Anti-IL-1 Receptor II>

文献数: 2

説明文 別名:beta
クローン:34141
Genbank No: 7850
Protein Accession No: P27930
法規制等
保存条件 4℃,暗所保存,凍結禁止 法規備考
抗原種 免疫動物 Mouse
交差性 Human 適用 FCM
標識 PE 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 MagCellect™ Mouse and Human Natural Killer Cell Isolation Kits
M1/M2 Macrophage Activation Marker
関連記事



目次に戻る

Product Details

Species ReactivityHuman
LabelPhycoerythrin
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑1 RIIPhe14-Glu343 (Ser56Gly and Glu297Gly)Accession # P27930
SourceMonoclonal Mouse IgG1 Clone # 34141
PurificationProtein A or G purified from ascites
SpecificityDetects human IL-1 RII in direct ELSAs and Western blots. When used in combination with the biotinylated human IL-1 RII affinity purified polyclonal detection antibody (Catalog # BAF263) in sandwich ELISAs, no significant cross-reactivity or interference was observed with recombinant human (rh) IL-1ra, rhIL-1 RI,  recombinant mouse IL-1ra,  or recombinant rat IL-1ra.


目次に戻る

Applications and Data

 Recommended
Concentration
Sample
Flow Cytometry10 µL/106 cellsSee below


Flow Cytometry
Detection of IL‑1 RII in Human PBMCs stimulated to induce Tregs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs), stimulated to induce Regulatory T Cells (Tregs), were treated with 10 ug/mL Anti-CD3, 5 ug/mL Anti-CD28, 10 ng/mL Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and 20 ng/mL Recombinant Human IL‑2 (Catalog # 202-IL) for 48 hours and stained with Rat Anti-Human LRRC32/GARP APC‑conjugated Monoclonal Antibody and either (A) Mouse Anti-Human IL‑1 RII PE‑conjugated Monoclonal Antibody (Catalog # FAB663P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). Cells were gated on CD4.  View our protocol for Staining Membrane-associated Proteins.


目次に戻る

Related Product & Information

BackgroundIL-1 RII
background_contentBackground:
IL-1 RII
Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes, and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex. An IL-1 receptor accessory protein that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta, but not IL-1ra, was identified (1). This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.


目次に戻る

Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Phenotypic changes of peripheral blood mononuclear cells upon corticosteroid treatment in idiopathic intermediate uveitis
    Authors: Karoline Walscheid
    Clin. Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

  2. Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways.
    Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D
    J Immunol, 2015;195(6):2818-28.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow



目次に戻る

お問い合わせ先

(テクニカルサポート 試薬担当)

reagent@funakoshi.co.jp

製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。