抗Human IL-10 R alpha Phycoerythrin抗体(Anti-Human IL-10 R alpha Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑10 R alpha His22-Asn235Accession # Q13651
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL‑10 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant mouse IL-10 R alpha and less than 5% cross-reactivity with recombinant human (rh) CNTF sR alpha and rhIL-2 sR gamma is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of IL‑10 R alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Goat Anti-HumanIL‑10 R alpha PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6280P) or (B) Normal Goat IgG Phycoerythrin Control (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.

目次に戻る

Related Product & Information

Long Name	Interleukin 10 Receptor alpha
Background	IL-10 R alpha
background_content	Background: IL-10 R alpha IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), is a potent immunosuppressant of macrophage functions. IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. IL-10 binds specifically and with high affinity to cell-surface receptors. Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. The IL-10 R mRNA has been detected in all cell types that are known to respond to IL-10. Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3. Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist.

目次に戻る

Citations

1. cmvIL-10 stimulates the invasive potential of MDA-MB-231 breast cancer cells.
   Authors: Valle Oseguera, Cendy A, Spencer, Juliet V
   PLoS ONE, 2014;9(2):e88708.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

目次に戻る