抗GM-CSF Rα抗体(Anti-GM-CSF Rα, Rat-Mono, APC antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Allophycocyanin
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse GM-CSF R alpha Leu30-Pro327Accession # Q00941
Source	Monoclonal Rat IgG2A Clone # 698423
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse GM-CSF R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) GM-CSF R alpha or rhGM‑CSF R beta is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of GM‑CSF R alpha in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse GM‑CSF R alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB6130A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

GM-CSF R alpha

background_content

Background: GM-CSF R alpha Granulocyte macrophage colony stimulating factor receptor alpha (GM-CSF R alpha ), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM-CSF. GM-CSF promotes the differentiation and mobilization of granulocyte-macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effector functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1-4). Mature mouse GM-CSF R alpha is an 80 kDa type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains and a juxtamembrane WSXWS motif, a 21 aa transmembrane segment, and a 40 aa cytoplasmic domain (5). Within the ECD, mouse GM-CSF R alpha shares approximately 33% and 58% aa sequence identity with human and rat GM-CSF R alpha, respectively. Soluble forms of the human receptor retain the ability to bind GM-CSF (6, 7). GM-CSF R alpha is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non‑hematopoietic solid tumor cells (8). GM-CSF R alpha associates with the common beta chain/CD131 ( beta c), a 135 kDa transmembrane protein that is also the signal transducing component of the receptors for IL-3 and IL-5 (9, 10). Association with beta c converts GM-CSF R alpha from a low affinity to a high affinity receptor for GM-CSF (9-11). The shared usage of beta c underlies the synergism between GM-CSF, IL-3, and IL-5 in their effects on myeloid cell differentiation and activation (1, 2).

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Citations

1. Trogocytosis of peptide-MHC class II complexes from dendritic cells confers antigen-presenting ability on basophils
   Authors: K Miyake, N Shiozawa, T Nagao, S Yoshikawa, Y Yamanishi, H Karasuyama
   Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Transgenic expression of GM-CSF in T cells causes disseminated histiocytosis.
   Authors: van Nieuwenhuijze A, Coghill E, Gray D, Prato S, Metcalf D, Alexander W, Wicks I
   Am J Pathol, 2014;184(1):184-99.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. GM-CSF-licensed CD11b+ lung dendritic cells orchestrate Th2 immunity to Blomia tropicalis.
   Authors: Zhou Q, Ho A, Schlitzer A, Tang Y, Wong K, Wong F, Chua Y, Angeli V, Mortellaro A, Ginhoux F, Kemeny D
   J Immunol, 2014;193(2):496-509.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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