抗Mouse Neuropilin-1 Alexa Fluor 700抗体(Anti-Mouse Neuropilin-1 Alexa Fluor 700 antibody)

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• Product Details
• Applications and Data
• Related Product & Information

価格

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Product Details

Species Reactivity	Mouse
Label	Alexa Fluor 700
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Neuropilin-1Phe21-Pro856 Accession # P97333
Source	Monoclonal Rat IgG2A Clone # 761705
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse Neuropilin-1 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant rat (rr) Neuropilin-1 is observed and no cross-reactivity with recombinant human (rh) Neuropilin-1, rhNeuropilin-2, or rrNeuropilin-2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	5 µL/106 cells	See below

Flow Cytometry
	Detection of Neuropilin‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Neuropilin-1 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB5994N, filled histogram) or isotype control antibody (Catalog # IC006N, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

Neuropilin-1

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Background: Neuropilin-1 Neuropilin-1 (Nrp-1, previously neuropilin; also CD304) is a 130 - 140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1 - 4). The full-length 923 amino acid (aa) mouse Nrp-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with rat and 93% aa identity with human, equine, bovine and canine Nrp-1 (3, 4). The ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). At least one splice variant that diverges at aa 587 and lacks the TM domain has been sequenced (5). This form is potentially a soluble antagonist, based on results from human Nrp-1 splice variants (1, 6 - 8). The sema domains of Class III secreted semaphorins such as Sema3A bind Nrp-1 a1a2 (9). Heparin, the heparin-binding forms of VEGF (VEGF165, VEGF-B and VEGF-E), PlGF (PlGF2), and the C-terminus of Sema3 bind the b1b2 region (9, 10). Nrp-1 and Nrp-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (11). However, only Nrp-1 binds Sema3A, and only Nrp-2 binds Sema3F (1). Both are co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF165 binding (1). Sema3A signaling can be blocked by VEGF165, which has higher affinity for Npn-1 (12). Nrp-1 is preferentially expressed in developing or remodeling arteries (1, 2). Nrp-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (13).

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