抗MGL1/2抗体(Anti-MGL1/2, Goat-Poly, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse MGL1/2 (CD301a/b)Gln57-Ser304Accession # AAH14811
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse MGL1 (CD301a) and MGL2 (CD301b) in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of MGL1/2 (CD301a/b) in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse MGL1/2 (CD301a/b) PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB4297P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

MGL1/2 (CD301a/b)

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Background: MGL1/2 (CD301a/b) Mouse MGL1 (macrophage galactose N-acetyl-galactosamine (GalNAc) specific Lectin 1, CD301a), also called ASGP-BP (asialoglycoprotein binding protein), is a 38 kDa type II transmembrane glycoprotein of the C-type lectin family (1). Two MGL proteins are encoded by separate genes in the mouse, but share 91% amino acid (aa) identity in the extracellular domain (ECD) (2). Only one MGL occurs in human and rat, and this is more structurally similar to mouse MGL1 than MGL2. However, mouse MGL1 binds Lewis X, in contrast to human MGL and mouse MGL2 which both bind specifically to terminal GalNAc residues (2). Lewis X is a trisaccharide commonly found on leukocytes and some tumor cells. Both mouse MGL proteins are expressed on immature dendritic cells. Mouse MGL1 and MGL2 are markers for connective tissue macrophages of a type termed alternately activated macrophages. These macrophages are induced by IL-4 that is produced during Th2-mediated inflammatory responses to parasitic infections or allergic airway inflammation (3, 4). Quantitative RT-PCR after helminth infection shows a peak of MGL1 expression at 7 days, while MGL2 shows increasing expression for at least 29 days (3). This, and data from MGL1 knockout mice (5), indicates that MGL1 is critical during the formation of granulation tissue, with MGL2 remaining involved during chronic infection. Mouse MGL1 is synthesized with an N-terminal 35 aa cytoplasmic region, a 21 aa transmembrane segment and a 248 aa ECD. The ECD contains one 129 aa carbohydrate recognition domain (CRD) that shows 78% and 63% aa identity with rat and human MGL, respectively.

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Citations

1. CCR2(-) and CCR2(+) corneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion
   Authors: J Liu, Y Xue, D Dong, C Xiao, C Lin, H Wang, F Song, T Fu, Z Wang, J Chen, H Pan, Y Li, D Cai, Z Li
   Mucosal Immunol, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Akt2 deficiency protects from acute lung injury via alternative macrophage activation and miR-146a induction in mice.
   Authors: Vergadi, Eleni, Vaporidi, Katerina, Theodorakis, Emmanuel, Doxaki, Christin, Lagoudaki, Eleni, Ieronymaki, Elefther, Alexaki, Vassilia, Helms, Mike, Kondili, Eumorfia, Soennichsen, Birte, Stathopoulos, Efstathi, Margioris, Andrew N, Georgopoulos, Dimitrio, Tsatsanis, Christos
   J Immunol, 2014;192(1):394-406.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. Obesity alters adipose tissue macrophage iron content and tissue iron distribution.
   Authors: Orr J, Kennedy A, Anderson-Baucum E, Webb C, Fordahl S, Erikson K, Zhang Y, Etzerodt A, Moestrup S, Hasty A
   Diabetes, 2014;63(2):421-32.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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