抗Mouse Nectin-2/CD112 Phycoerythrin抗体(Anti-Mouse Nectin-2/CD112 Phycoerythrin antibody)

掲載日情報:2018/11/26 現在Webページ番号:195525

Mouse Nectin-2/CD112 Phycoerythrinに対する抗体(Anti-Mouse Nectin-2/CD112 Phycoerythrin )です。
本製品は研究用です。研究用以外には使用できません。

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納期 文献数
Anti-Mouse Nectin-2/CD112 Phycoerythrin MAb (Clone 829038)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
別名:CD112
クローン:829038
Genbank No: 5819
Protein Accession No: P32507
法規制等
保存条件 4℃,暗所保存,凍結禁止 法規備考
抗原種 免疫動物 Rat クラス IgG 標識 PE
交差性 Mouse 適用 FCM
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 GMP-grade Recombinant Human FGF basic / PAPS Synthesis Kit
関連記事

[在庫・価格 :2024年04月29日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Mouse Nectin-2/CD112 Phycoerythrin MAb (Clone 829038)

文献数: 0

説明文 別名:CD112
クローン:829038
Genbank No: 5819
Protein Accession No: P32507
法規制等
保存条件 4℃,暗所保存,凍結禁止 法規備考
抗原種 免疫動物 Rat
交差性 Mouse 適用 FCM
標識 PE 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 GMP-grade Recombinant Human FGF basic / PAPS Synthesis Kit
関連記事



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Product Details

Species ReactivityMouse
LabelPhycoerythrin
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Nectin-2/CD112 Gln32-Gly351 (predicted)Accession # P32507
SourceMonoclonal Rat IgG2A Clone # 829038
PurificationProtein A or G purified from hybridoma culture supernatant
SpecificityDetects mouse Nectin-2/CD112 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Nectin-2 or recombinant mouse CD155/PVR is observed.


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Applications and Data

 Recommended
Concentration
Sample
Flow Cytometry10 µL/106 cellsSee below


Flow Cytometry
Detection of Nectin‑2/CD112 in C2C12 Mouse Cell Line by Flow Cytometry.
C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Nectin‑2/CD112 PE‑conjugated Monoclonal Antibody (Catalog # FAB3869P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.


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Related Product & Information

BackgroundNectin-2/CD112
background_contentBackground:
Nectin-2/CD112
Nectins are a small family of Ca++-independent immunoglobulin (Ig)-like Cell Adhesion Molecules (CAMs) that control cell adhesion, proliferation, and migration (1, 2, 3). The name Nectin derives from the Latin word necto, which means “to connect”. The Nectin family contains four members (Nectin-1 to -4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma  which is secreted), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such have been given the alternate name of poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus (1). Mouse Nectin-2 is a 70 to 78 kDa type I TM glycoprotein that is found on a variety of cell types (4, 5). It has two splice forms (4, 6, 7). Nectin-2 alpha /PRR2 is a 65 kDa short form and is synthesized as a 467 amino acid precursor. It contains a 31 aa signal sequence, a 315 aa extracellular domain (ECD), a 28 aa TM segment, and a 93 aa cytoplasmic region. The ECD contains one N-terminal V-type Ig domain and two 85-95 aa C2-type Ig-like domains (aa 153-337) (8). The V-domain is believed to mediate Nectin binding to its ligands (9). A long, 78 kDa, 530 aa isoform of mouse Nectin-2 (Nectin-2δ) also exists. It has the same signal sequence and extracellular domain as Nectin-2 alpha  (aa 1-338), but differs in the TM segment (21 aa in length) and cytoplasmic region (159 aa in length) (4, 6, 7). Mouse Nectin-2 ECD (aa 32-338) shares 72%, 77% and 95% aa identity with the ECD in human, canine and rat Nectin-2, respectively. Nectin-2 is known to bind pseudorabies virus, and herpes simplex virus-2 (HSV-2). It also binds select HSV-1 strains. It does not bind poliovirus (1, 10, 11). As a cell adhesion molecule, Nectin-2 will form cis-homodimers (same cell) and trans-homodimers (across cells). Nectin-2 will not cis-dimerize with other Nectins, but will trans-heterodimerize with Nectin-3 and CD266/DNAM-1 (1, 3, 11, 12, 13). Nectin-2 is found concentrated at cell-to-cell interfaces, and is presumed to contribute to tight and adherens junction formation (14). Through its interaction with NK and T cell expressed DNAM-1, it also promotes lymphocyte cytotoxicity and cytokine secretion against both tumors and dendritic cells (DC) expressing Nectin-2 (15, 16). In the case of DC, this may be a mechanism whereby the immune system eliminates DC that are inefficient at antigen presentation. Nectin-2 is expressed on epithelium, endothelial cells, Sertoli cells, monocytes, dendritic cells, granulosa cells, mast cells, eosinophils and fibroblasts.


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