抗Human SR-AI/MSR1 Phycoerythrin抗体(Anti-Human SR-AI/MSR1 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human SR‑AI/MSRLys77-Leu451Accession # P21757
Source	Monoclonal Mouse IgG2B Clone # 351615
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human SR‑AI/MSR in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse SR-AI is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of SR‑AI/MSR in THP‑1 Human Cell Line by Flow Cytometry. THP‑1 human acute monocytic leukemia cell line activated with PMA and Ca2+ ionomycin was stained with Mouse Anti-Human SR‑AI/MSR PE-conjugated Monoclonal Antibody (Catalog # FAB2708P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

SR-AI/MSR

background_content

Background: SR-AI/MSR The type I class A macrophage scavenger receptor (SR-AI; also MSR-AI) is a 70-80 kDa protein that belongs to the scavenger receptor superfamily (1‑3). Receptors of this family contain characteristic extracellular domains and bind to a series of generally unrelated, but negatively-charged/polyanionic ligands (1, 3). Human SR-AI is a type II transmembrane glycoprotein that is 451 amino acids (aa) in length. It contains a 50 aa cytoplasmic tail, a 26 aa transmembrane segment and a 375 aa extracellular region (4, 5). The extracellular region contains four definitive domains, with a membrane proximal spacer of 33 aa, an alpha -helical coiled-coil domain of 163 aa, a collagen-like domain of 69 aa, and a cysteine-rich C-terminus of 110 aa (4, 6). The cysteine-rich domain (CRD) forms three intrachain disulfide bonds (7). The functional form of the molecule is a 220‑230 kDa membrane-associated trimer that, in human, apparently has two disulfide bonded chains and a third noncovalently associated subunit (8, 9). Human extracellular region is 73% and 72% aa identical to bovine and mouse SR-AI extracellular region, respectively. The human gene for SR-A gives rise to three isoforms; the I isoform of 451 aa, the II isoform of 358 aa, and the III isoform of 388 aa (4, 5, 10). All are identical through the first 344 aa which includes the cytoplasmic tail through the collagenous domain. Isoform II (SR-AII) shows a severe truncation of the CRD, but is expressed on the cell surface. Isoform III (SR-AIII) has a modest truncation of the CRD, and cannot be expressed on the cell surface. However, relative to SR-AI, SR-AII is known to show differential sensitivity to LPS and receptor binding to gram‑negative bacteria (9, 11), while SR-AIII is known to be a dominant-negative isoform (10). SR-AIII may achieve this by either heterotrimerizing with SR-AI, or simply eliminating the production of SR-AI mRNA.

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Citations

1. Imatinib and Nilotinib Off-Target Effects on Human NK Cells, Monocytes, and M2 Macrophages
   Authors: F Bellora, A Dondero, MV Corrias, B Casu, S Regis, F Caliendo, A Moretta, M Cazzola, C Elena, L Vinti, F Locatelli, C Bottino, R Castriconi
   J. Immunol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Oxidized LDL triggers changes in oxidative stress and inflammatory biomarkers in human macrophages
   Authors: OJ Lara-Guzmá, Á Gil-Izquie, S Medina, E Osorio, R Álvarez-Qu, N Zuluaga, C Oger, JM Galano, T Durand, K Muñoz-Dura
   Redox Biol, 2017;15(0):1-11.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Combination of plasma HA and circulating M2-like monocytes may serve as a diagnostic marker for breast cancer
   Authors: B Zhang, M Cao, Y He, Y Liu, G Zhang, C Yang, Y Du, J Xu, J Hu, F Gao
   J Cancer, 2017;8(17):3522-3530.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. The Impact of Protein Corona Formation on the Macrophage Cellular Uptake and Biodistribution of Spherical Nucleic Acids
   Authors: AB Chinen, CM Guan, CH Ko, CA Mirkin
   Small, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Increased circulating M2-like monocytes in patients with breast cancer
   Authors: B Zhang, M Cao, Y He, Y Liu, G Zhang, C Yang, Y Du, J Xu, J Hu, F Gao
   Tumour Biol., 2017;39(6):1010428317711.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Oxidative stress decreases functional airway mannose binding lectin in COPD.
   Authors: Tran, Hai B, Ahern, Jessica, Hodge, Greg, Holt, Phillip, Dean, Melinda, Reynolds, Paul N, Hodge, Sandra
   PLoS ONE, 2014;9(6):e98571.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Effect of apoptotic cell recognition on macrophage polarization and mycobacterial persistence.
   Authors: de Oliveira Fulco T, Andrade P, de Mattos Barbosa M, Pinto T, Ferreira P, Ferreira H, da Costa Nery J, Real S, Borges V, Moraes M, Sarno E, Sampaio E, Pinheiro R
   Infect Immun, 2014;82(9):3968-78.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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