抗Mouse MULT-1 Phycoerythrin抗体(Anti-Mouse MULT-1 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Phycoerythrin
Immunogen	BaF3 mouse pro-B cell line transfected with mouse ULBP‑1 /MULT-1
Source	Monoclonal Rat IgG2A Clone # 237104
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse ULBP‑1 /MULT-1 in flow cytometry.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of ULBP‑1 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse ULBP‑1 /MULT-1 PE‑conjugated Monoclonal Antibody (Catalog # FAB2588P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

ULBP-1

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Background: ULBP-1 ULBP-1, also known as MULT-1 (Mouse UL16-Binding Protein-like Transcript 1), is a 53 kDa, MHC Class I-like molecule that belongs to the mouse family of NKG2D ligands (1‑4). It is a type I transmembrane glycoprotein that is synthesized as a 334 amino acid (aa) precursor. It contains a 25 aa signal sequence, a 186 aa extracellular region, a 19 aa transmembrane segment and a 104 aa cytoplasmic tail (2). The extracellular region contains an alpha 1 and alpha 2 like domain with two intrachain disulfide bonds. ULBP-1 is distantly related to other human and mouse NKG2D ligands, and more distantly related to the MHC class I proteins (3). Unlike most NKG2D ligands, transcripts for ULBP-1 have been detected in a wide variety of mouse tissues and tumor cells lines (3). The receptor for ULBP-1 is NKG2D, a 35 kDa C-type lectin that is found on mouse NK cells, activated CD8+ T cells, epidermal gamma δ T cells, and activated macrophages (1, 5, 6, 7). Recombinant ULBP-1 protein binds to NKG2D with high affinity (KD = 6 nM) (2). Although an activating receptor, general cellular responses to NKG2D ligation depend upon the isoform of NKG2D and the cell type (5). Exposure to immobilized ULBP-1 or ULBP-1-transfected cells elicits IFN-gamma production by NK cells (3). Ectopic expression of ULBP-1 on the RMA mouse tumor cell line leads to tumor rejection in syngeneic mice (3).

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Citations

1. NKG2D-NKG2D Ligand Interaction Inhibits the Outgrowth of Naturally Arising Low-Grade B Cell Lymphoma In Vivo
   J Immunol, 2016;196(11):4805-13.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Toll-Like receptor 2 (TLR2) and TLR9 play opposing roles in host innate immunity against Salmonella enterica serovar Typhimurium infection.
   Authors: Zhan R, Han Q, Zhang C, Tian Z, Zhang J
   Infect Immun, 2015;83(4):1641-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. Role of CC chemokine receptor 4 in natural killer cell activation during acute cigarette smoke exposure.
   Authors: Stolberg V, Martin B, Mancuso P, Olszewski M, Freeman C, Curtis J, Chensue S
   Am J Pathol, 2014;184(2):454-63.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen

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