抗TIM-3抗体(Anti-TIM-3, Rat-Mono, Alexa Fluor 700 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Alexa Fluor 700
Immunogen	Mouse myeloma cell line NS0-derived recombinant human TIM‑3Ser22-Arg200Accession # Q8TDQ0.2
Source	Monoclonal Rat IgG2A Clone # 344823
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	5 µL/106 cells	See below

Flow Cytometry

Detection of TIM‑3 in Human PBMCs by Flow Cytometry. Human peripheral blood mono­nuclear cells (PBMCs) were stained with Rat Anti-Human TIM‑3 Alexa Fluor® 700‑conjugated Mono­clonal Antibody (Catalog # FAB2365N) and Mouse Anti-Human CD14 PE‑conjugated Mono­clonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # IC006N). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

TIM-3

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Background: TIM-3 TIM-3 (T cell immunoglobulin and mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). There are three TIM genes in human and eight in mouse. Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4+ Th1 and CD8+ Tc1) but not on helper T cells (CD4+ Th2 and CD8+ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. It also occurs on hippocampal neurons (7-10). The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 11). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and proapoptotic signaling (8, 12). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 10, 11, 13), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (14). Soluble mouse TIM-3 constructs which lack the cytoplasmic domain have been shown to inhibit anti-tumor effector T cell responses and to enhance autoimmune reactions (5, 15).

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Citations

1. Effect of IL-7 Therapy on Phospho-Ribosomal Protein S6 and TRAF1 Expression in HIV-Specific CD8 T Cells in Patients Receiving Antiretroviral Therapy
   Authors: C Wang, MI Edilova, LE Wagar, S Mujib, M Singer, NF Bernard, T Croughs, MM Lederman, I Sereti, MA Fischl, E Kremmer, M Ostrowski, JP Routy, TH Watts
   J. Immunol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Increased frequency of Tim-3 expressing T cells is associated with symptomatic West Nile virus infection.
   Authors: Lanteri M, Diamond M, Law J, Chew G, Wu S, Inglis H, Wong D, Busch M, Norris P, Ndhlovu L
   PLoS ONE, 2014;9(3):e92134.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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