抗TIM-3抗体(Anti-TIM-3, Rat-Mono, APC antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Allophycocyanin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human TIM‑3Ser22-Arg200Accession # Q8TDQ0.2
Source	Monoclonal Rat IgG2A Clone # 344823
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of TIM‑3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human TIM‑3 APC‑conjugated Monoclonal Antibody (Catalog # FAB2365A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

TIM-3

background_content

Background: TIM-3 TIM-3 (T cell Immunoglobulin and Mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4+ Th1 and CD8+ Tc1) but not on helper T cells (CD4+ Th2 and CD8+ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 6). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and proapoptotic signaling (7). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 6, 8), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (9).

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Citations

1. Circulating T lymphocyte subsets, cytokines, and immune checkpoint inhibitors in patients with bipolar II or major depression: a preliminary study
   Authors: W Wu, YL Zheng, LP Tian, JB Lai, CC Hu, P Zhang, JK Chen, JB Hu, ML Huang, N Wei, WJ Xu, WH Zhou, SJ Lu, J Lu, HL Qi, DD Wang, XY Zhou, JF Duan, Y Xu, SH Hu
   Sci Rep, 2017;7(0):40530.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow Ctyometry


2. T-cell invigoration to tumour burden ratio associated with anti-PD-1 response
   Authors: AC Huang, MA Postow, RJ Orlowski, R Mick, B Bengsch, S Manne, W Xu, S Harmon, JR Giles, B Wenz, M Adamow, D Kuk, KS Panageas, C Carrera, P Wong, F Quagliarel, B Wubbenhors, K D'Andrea, KE Pauken, RS Herati, RP Staupe, JM Schenkel, S McGettigan, S Kothari, SM George, RH Vonderheid, RK Amaravadi, GC Karakousis, LM Schuchter, X Xu, KL Nathanson, JD Wolchok, TC Gangadhar, EJ Wherry
   Nature, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. TIM-3/Gal-9 interaction induces IFNgamma-dependent IDO1 expression in acute myeloid leukemia blast cells.
   Authors: Folgiero V, Cifaldi L, Li Pira G, Goffredo B, Vinti L, Locatelli F
   J Hematol Oncol, 2015;8(0):36.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Inhibitory phenotype of HBV-specific CD4+ T-cells is characterized by high PD-1 expression but absent coregulation of multiple inhibitory molecules.
   Authors: Raziorrouh B, Heeg M, Kurktschiev P, Schraut W, Zachoval R, Wendtner C, Wachtler M, Spannagl M, Denk G, Ulsenheimer A, Bengsch B, Pircher H, Diepolder H, Gruner N, Jung M
   PLoS ONE, 2014;9(8):e105703.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Peripheral blood TIM-3 positive NK and CD8+ T cells throughout pregnancy: TIM-3/galectin-9 interaction and its possible role during pregnancy.
   Authors: Meggyes, Matyas, Miko, Eva, Polgar, Beata, Bogar, Barbara, Farkas, Balint, Illes, Zsolt, Szereday, Laszlo
   PLoS ONE, 2014;9(3):e92371.
   Species: Human
   Sample Type:
   


6. Differential regulation of interleukin-12 (IL-12)/IL-23 by Tim-3 drives T(H)17 cell development during hepatitis C virus infection.
   Authors: Wang, Jia M, Shi, Lei, Ma, Cheng J, Ji, Xiao J, Ying, Ruo S, Wu, Xiao Y, Wang, Ke S, Li, Guangyu, Moorman, Jonathan, Yao, Zhi Q
   J Virol, 2013;87(8):4372-83.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Blockade of Tim-3 signaling restores the virus-specific CD8+ T-cell response in patients with chronic hepatitis B.
   Authors: Wu W, Shi Y, Li S, Zhang Y, Liu Y, Wu Y, Chen Z
   Eur. J. Immunol., 2012;42(5):1180-91.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Tim-3 negatively regulates IL-12 expression by monocytes in HCV infection.
   Authors: Zhang Y, Ma CJ, Wang JM, Ji XJ, Wu XY, Jia ZS, Moorman JP, Yao ZQ
   PLoS ONE, 2011;6(5):e19664.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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