抗Mouse LYVE-1 Phycoerythrin抗体(Anti-Mouse LYVE-1 Phycoerythrin antibody)

掲載日情報:2018/11/26 現在Webページ番号:195376

Mouse LYVE-1 Phycoerythrinに対する抗体(Anti-Mouse LYVE-1 Phycoerythrin )です。
本製品は研究用です。研究用以外には使用できません。

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Anti-Mouse LYVE-1 Phycoerythrin MAb (Clone 223322)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 4
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説明文
別名:cell surface retention sequence binding protein-1
クローン:223322
Genbank No: 10894
Protein Accession No: Q8BHC0
法規制等
保存条件 4℃,暗所保存 法規備考
抗原種 免疫動物 Rat クラス IgG 標識 PE
交差性 Mouse 適用 FCM
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
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[在庫・価格 :2024年06月16日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Mouse LYVE-1 Phycoerythrin MAb (Clone 223322)

文献数: 4

説明文 別名:cell surface retention sequence binding protein-1
クローン:223322
Genbank No: 10894
Protein Accession No: Q8BHC0
法規制等
保存条件 4℃,暗所保存 法規備考
抗原種 免疫動物 Rat
交差性 Mouse 適用 FCM
標識 PE 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事
関連記事



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Product Details

Species ReactivityMouse
LabelPhycoerythrin
ImmunogenBaF/3 mouse pro-B cell line transfected with mouse LYVE-1Ala24-Thr234Accession # Q8BHC0
SourceMonoclonal Rat IgG2A Clone # 223322
PurificationProtein A or G purified from hybridoma culture supernatant
SpecificityDetects mouse LYVE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD44 or recombinant human LYVE-1 is observed.


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Applications and Data

 Recommended
Concentration
Sample
Flow Cytometry10 µL/106 cellsSee below


Flow Cytometry
Detection of LYVE‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse LYVE‑1 PE‑conjugated Monoclonal Antibody (Catalog # FAB2125P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.


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Related Product & Information

BackgroundLYVE-1
background_contentBackground:
LYVE-1
Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1-3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity. LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9-11).


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Apelin modulates pathological remodeling of lymphatic endothelium after myocardial infarction
    Authors: F Tatin, E Renaud-Gab, AC Godet, F Hantelys, F Pujol, F Morfoisse, D Calise, F Viars, P Valet, B Masri, AC Prats, B Garmy-Susi
    JCI Insight, 2017;2(12):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Flow

  2. Gene-expression profiling of different arms of lymphatic vasculature identifies candidates for manipulation of cell traffic
    Proc Natl Acad Sci USA, 2016;113(38):10643-10648.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow



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