抗Human Semaphorin 7A Phycoerythrin抗体(Anti-Human Semaphorin 7A Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Semaphorin 7AHis47-Ala648Accession # O75326
Source	Monoclonal Mouse IgG2B Clone # 310829
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human Semaphorin 7A in direct ELISAs.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of Semaphorin 7A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PHA were stained with Mouse Anti-Human Semaphorin 7A PE‑conjugated Monoclonal Antibody (Catalog # FAB20681P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

Semaphorin 7A

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Background: Semaphorin 7A Semaphorin 7A (Sema7A, designated CD108, previously Sema K1 or Sema L), is an ~80 kDa membrane-anchored glycoprotein that is a member of the Semaphorin family of axon guidance molecules (1-4). On human erythrocytes, it is the John Milton Hagen (JMH) blood group antigen (4). Sema7A is the only known Class 7 or glycophosphatidylinositol (GPI)-linked semaphorin; its expression is concentrated in the brain, spleen and thymus (1-5). Human Sema7A cDNA encodes a 44 amino acid (aa) signal sequence, a 604 aa extracellular domain (ECD) including Sema and C2‑type Ig-like domains, and an 18 aa propeptide/GPI membrane anchor signal sequence. Mature human Sema7A shares 89%, 89%, 88%, 86% and 90% aa identity with mouse, rat, canine, bovine and equine Sema7A, respectively. The Sema7A sema domain contains an RGD integrin interaction motif (4). Although it binds plexin-C1 in vitro and may be coexpressed with it, many of its activities depend on interaction with beta 1 integrins such as alpha 1 beta 1 (6-10). Sema7A signaling through the two receptors may cause opposing effects (8). Sema7A is an immune semaphorin, with expression and activity on CD4+CD8+ thymocytes, activated T cells, macrophages and microglia (2, 9-12). T cell Sema7A interacts with monocytic cells, stimulating their chemotaxis, production of pro-inflammatory cytokines, and dendritic differentiation (5, 6). However, on the T cells themselves, Sema7A downregulates T cell Receptor (TCR) signaling by promoting TCR internalization, modulating T cell responses (9). In lung macrophages, Sema7A is induced by TGF-beta and participates in TGF-beta -induced lung fibrosis (12). Sema7A is also expressed on pre-osteoblasts and osteoclasts, where it promotes migration and fusion, respectively; on keratinocytes, where it promotes melanocyte spreading and dendricity; and on some neurons, for example, promoting axon outgrowth in the developing olfactory tract (8, 10, 13).

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