抗SR-AI抗体(Anti-SR-AI, Rat-Mono, APC antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Allophycocyanin
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse SR‑AI/MSRTrp79-Ser454Accession # AAA39747
Source	Monoclonal Rat IgG2B Clone # 268318
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse SR‑AI/MSR in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human SR-AI is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of SR‑AI/MSR in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse SR‑AI/MSR APC-conjugated Monoclonal Antibody (Catalog # FAB1797A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

SR-AI/MSR

background_content

Background: SR-AI/MSR The scavenger receptor (SR) family comprises a group of functionally defined membrane receptors that share the common ability to bind and internalize modified forms of Low Density Lipoproteins (mLDL) (1‑3). Family members are classified alphabetically. The A class include four proteins: the three subtypes of SR-A (AI, AII, and AIII) that are generated by alternative splicing of the same gene, and a structurally similar protein named MARCO (4). All A class SRs are multidomain trimeric type II membrane proteins. SR-AI has an N-terminal cytoplasmic domain, a transmembrane domain, a spacer domain, an alpha -helical coiled coil, a collagen-like domain and a C-terminal cysteine-rich domain. SR-A is expressed by most tissue macrophages, dendritic cells and Kupffer cells. It is also highly expressed by microglia in neonatal as well as Alzheimer’ Disease brains. SR-AI binds a broad range of polyanionic ligands including modified proteins (e.g. Oxidized, acetylated or maleylated LDL, Advanced glycation end-product proteins), polyribonucleotides (polyguanosine and polyinosine), polysaccharides (dextran sulfate, fucoidan), phospholipids (phosphatidylserine), bacterial products (lipopolysaccharide and lipoteichoic acid) and selected chemical compounds (silica, crocidolite asbestos). The ligand-binding region has been localized to a positively charged region in the carboxyl end of the collagen-like domain. Based on its ligand binding characteristics, SR-AI is implicated in many physiological and pathophysiological functions. Studies using SR-A knockout mouse have also suggested roles of SR-A in atherogenesis, host defense and innate immunity, acquired immune responses, macrophage adhesion, and phagocytosis of apoptotic cells (1‑3).

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Citations

1. Blockade of TGF-?/Smad signaling by the small compound HPH-15 ameliorates experimental skin fibrosis
   Authors: VH Luong, T Chino, N Oyama, T Matsushita, Y Sasaki, D Ogura, SI Niwa, T Biswas, A Hamasaki, M Fujita, Y Okamoto, M Otsuka, H Ihn, M Hasegawa
   Arthritis Res. Ther., 2018;20(1):46.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. KIT oncogene inhibition drives intratumoral macrophage M2 polarization.
   Authors: Cavnar, Michael, Zeng, Shan, Kim, Teresa S, Sorenson, Eric C, Ocuin, Lee M, Balachandran, Vinod P, Seifert, Adrian M, Greer, Jonathan, Popow, Rachel, Crawley, Megan H, Cohen, Noah A, Green, Benjamin, Rossi, Ferdinan, Besmer, Peter, Antonescu, Cristina, DeMatteo, Ronald P
   J Exp Med, 2013;210(13):2873-86.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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