抗Mouse OX40/TNFRSF4 Phycoerythrin抗体(Anti-Mouse OX40/TNFRSF4 Phycoerythrin antibody)

掲載日情報:2018/11/26 現在Webページ番号:195277

Mouse OX40/TNFRSF4 Phycoerythrinに対する抗体(Anti-Mouse OX40/TNFRSF4 Phycoerythrin )です。
本製品は研究用です。研究用以外には使用できません。

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Anti-Mouse OX40/TNFRSF4 Phycoerythrin Affinity Purified PAb
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 1
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説明文
別名:ACT-135
Genbank No: 7293
Protein Accession No: P47741
法規制等
保存条件 法規備考
抗原種 免疫動物 Goat クラス IgG 標識 PE
交差性 Mouse 適用 FCM
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
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[在庫・価格 :2025年07月01日 18時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Mouse OX40/TNFRSF4 Phycoerythrin Affinity Purified PAb

文献数: 1

説明文 別名:ACT-135
Genbank No: 7293
Protein Accession No: P47741
法規制等
保存条件 法規備考
抗原種 免疫動物 Goat
交差性 Mouse 適用 FCM
標識 PE 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事
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Product Details

Species ReactivityMouse
LabelPhycoerythrin
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse OX40/TNFRSF4Val20-Pro211Accession # P47741
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects mouse OX40/TNFRSF4 in direct ELISAs and Western blots. In these formats, less than 2% cross-reactivity with recombinant mouse (rm) EDAR, rm4-BB, rmCD27, rmDR3, rmGITR, rmNGF R, rmCD30, rmCD40, rmFas, rmOPG, rmRANK, rmTNF RI, and rmTNF RII is observed.


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Applications and Data

 Recommended
Concentration
Sample
Flow Cytometry10 µL/106 cellsSee below


Flow Cytometry
Detection of OX40/TNFRSF4 in Mouse Splenocytes by Flow Cytometry.
Activated-mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Goat Anti-Mouse OX40/TNFRSF4 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1256P) or (B) Normal Goat IgG Phycoerythrin Control (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.


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Related Product & Information

BackgroundOX40/TNFRSF4
background_contentBackground:
OX40/TNFRSF4
OX40, also known as CD134, was originally identified as an activated rat CD4+ T cell-surface antigen that is recognized by the monoclonal antibody MRC OX40. It is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF4. Mouse OX40 cDNA encodes a 256 amino acid (aa) residues type I transmembrane precursor protein with a putative 19 aa signal peptide, a 192 aa extracellular domain containing 4 TNFR-cysteine rich repeats, a 25 aa transmembrane domain and a 36 aa cytoplasmic region. A naturally occurring soluble OX40 has also been identified in human serum. Mouse OX40 shares approximately 63% and 90% aa sequence identity with its human and rat counterparts, respectively. OX40 is a T cell activation antigen that is expressed primarily on activated CD4+ T cells, but is also expressed on activated human and mouse CD8+ T cells. The ligand of OX40 is OX40 Ligand (OX40L), also known as gp34, a type II transmembrane glycoprotein belonging to the TNF superfamily. OX40L is expressed on activated B cells, T cells, dendritic cells and endothelial cells. Ligation of OX40 on T cells by OX40L or an agonistic antibody can promote clonal expansion, long-term T cell survival, and enhance memory T cell development. In vivo, blockade of OX40/OX40L interaction has been useful for treating autoimmune disease and graft-versus-host disease in animal models. Activation of OX40 has also been utilized to enhance the potency of vaccines and augment anti-tumor immunity (1-9).


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Crucial role of OX40/OX40L signaling in a murine model of asthma
    Authors: W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang
    Mol Med Rep, 2018;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow



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