抗Drosophila Smad2抗体(Anti-Drosophila Smad2 antibody)
掲載日情報：2018/11/26 現在Webページ番号：195132
Drosophila Smad2に対する抗体(Anti-Drosophila Smad2 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2025年06月06日 12時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
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文献数 | ||
|---|---|---|---|---|---|
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Anti-Drosophila Smad2 Affinity Purified Polyclonal Ab |
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Anti-Drosophila Smad2 Affinity Purified Polyclonal Ab |
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0 | |||
[在庫・価格 ：2025年06月06日 12時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Drosophila Smad2 Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF7948
- メーカー：RSD
- 包装：100μg
- 価格：¥100,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Drosophila Smad2 Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF7948-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Drosophila |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant Drosophila Smad2Gly262-Ser486Accession # O96660 |
| Source | Polyclonal Sheep IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects Drosophila Smad2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Smad1 is observed, and less than 1% cross-reactivity with rhSmad2, rhSmad3, and rhSmad5 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1-0.7 µg/mL | See below |
| Immunohistochemistry | 0.1-0.7 µg/mL | See below |
| Western Blot | |
|---|---|
 | Detection of Drosophila Smad2 by Western Blot. Western blot shows recombinant Drosophila Smad2 (2 ng/lane). PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Drosophila Smad2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7948) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Smad2 (C-terminal fragment) at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Western Blot | |
 | Detection of Drosophila Smad2 by Western Blot. Western blot shows wild-type and Smad2 null mutation Drosophila larval extracts. PVDF membrane was probed with 0.7 µg/mL of Sheep Anti-Drosophila Smad2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7948) followed by Anti-Sheep IgG Secondary Antibody. A specific band was detected for Smad2 at the expected mobility of approximately 58 kDa, but not mutant extracts. This experiment was conducted under reducing conditions. dSmad2 null mutation is described in Peterson, AJ, et al. (2012) PLoS One 7: e36548. Image courtesy of Dr. Aidan Peterson and Dr. Michael O'Connor, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA. |
| Immunohistochemistry | |
 | Smad2 in Drosophila Larvae. Smad2 was detected in wild-type and Smad2 null mutation Drosophila larvae using Sheep Anti-Drosophila Smad2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7948) at 0.7 μg/mL. Tissue was stained using a fluorescent Anti-Sheep IgG Secondary Antibody. A single confocal image in the upper panel depicts detection of endogenous Smad2 in epidermal and central nervous system cells. The dSmad2 mutant embryo in the lower panel was stained and imaged identically and reveals background staining. dSmad2 null mutation is described in Peterson, AJ, et al. (2012) PLoS One 7: e36548. Image courtesy of Dr. Aidan Peterson and Dr. Michael O'Connor, Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA. |
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Related Product & Information
| Long Name | Mothers Against DPP Homolog 2 |
|---|---|
| Background | Smad2 |
| background_content | Background: Smad2 Smad2 (SMAll body plus Mothers Against Decapentaplegic; also SmoX [Smad on Chr X]) is a 54 kDa (predicted) member of the receptor regulated Smad family of proteins. It is a downstream component of the Drosophila activin/TGF beta signaling pathway, and appears to play a key role in cell proliferation. Analogous to the system in vertebrates, stimulation of the fruitfly activin type I receptor (baboon):type II receptor (punt) complex results in dSmad2 (Drosophila Smad2) phosphorylation and subsequent interaction with dSmad4/Medea. As in vertebrates, the Drosophila Samd2/Smad4 complex enters the nucleus and interacts with a transcriptional cofactor(s) termed TGIF. At this point, the vertebrate:invertebrate systems diverge over the nature of the TGIF cofactor(s); vertebrate TGIF is a corepressor, while invertebrate TGIF (dTGIF) is a coactivator. Drosophila Smad2 is 486 amino acids (aa) in length. It contains two Mad homology domains (aa 7-130 and 285-475), the former of which participates in DNA-binding. Over aa 262-486, Drosophila Smad2 shares 70% aa sequence identity with both mouse and human Smad2. |
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