抗Human Ki-67/MKI67抗体(Anti-Human Ki-67/MKI67 antibody)
掲載日情報：2018/11/26 現在Webページ番号：195072
Human Ki-67/MKI67に対する抗体(Anti-Human Ki-67/MKI67 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2025年06月25日 17時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
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文献数 | ||
|---|---|---|---|---|---|
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Anti-Human Ki-67/MKI67 Affinity Purified Polyclonal Ab |
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3 | |||
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Anti-Human Ki-67/MKI67 Affinity Purified Polyclonal Ab |
|
1 | |||
[在庫・価格 ：2025年06月25日 17時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human Ki-67/MKI67 Affinity Purified Polyclonal Ab
文献数： 3
- 商品コード：AF7617
- メーカー：RSD
- 包装：100μg
- 価格：¥106,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human Ki-67/MKI67 Affinity Purified Polyclonal Ab
文献数： 1
- 商品コード：AF7617-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human Ki-67/MKI67Asn3120-Ile3256Accession # P46013 |
| Source | Polyclonal Sheep IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human Ki-67/MKI67 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Ki-67/MKI67 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Immunohistochemistry | 5-15 µg/mL | See below |
| Immunocytochemistry | 5-15 µg/mL | See below |
| Knockout Validated | Ki-67/MKI67 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Ki-67/MKI67 knockout HeLa cell line. | |
| Immunocytochemistry | |
|---|---|
 | Ki-67/MKI67 in A549 Human Cell Line. Ki-67/MKI67 was detected in immersion fixed A549 human lung carcinoma cell line using Sheep Anti-Human Ki-67/MKI67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7617) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei and nucleoli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Immunohistochemistry | |
 | Ki-67/MKI67 in Human Breast Cancer Tissue. Ki-67/MKI67 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human Ki-67/MKI67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7617) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
| Knockout Validated | |
 | Ki-67/MKI67 Specificity is Shown by Immunocytochemistry in Knockout Cell Line. Ki-67/MKI67 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in Ki-67/MKI67 knockout (KO) HeLa cell line using Sheep Anti-Human Ki-67/MKI67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7617) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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Related Product & Information
| Long Name | Antigen Identified by Monoclonal Antibody Ki-67 |
|---|---|
| Background | Ki-67/MKI67 |
| background_content | Background: Ki-67/MKI67 MKI67 (also Ki-67) is a 350-400 kDa nuclear protein that belongs to a molecular group comprised of mitotic chromosome-associated proteins. Ki-67 was originally recognized as an antigen associated with the monoclonal Ki-67 antibody raised against Hodgkin's lymphoma nuclear material. Ki-67 is contextually expressed, being potentially found in all cells that are not in the Go phase of the cell cycle. Thus, MKI67 qualifies as a cell proliferation marker. Functionally, Ki-67 is known to interact with 160 kDa Hklp2, a protein that promotes centrosome separation and spindle bipolarity. It also directly interacts with NIFK, and apparently binds to UBF, thus playing a role in rRNA synthesis. Human MKI67 is 3256 amino acids (aa) in length. It contains one FHA domain (aa 8-98), followed by at least 24 utilized Ser/Thr phosphorylation sites and sixteen 120 aa repeats (aa 1000-2928) that are interspersed with at least 90 additional utilized phosphorylation sites. There are two potential isoform variants. One isoform is 315-345 kDa in size and shows a deletion of aa 136-495, while a second isoform contains a 58 aa substitution for aa 1-513. Over aa 3120-3256, human Ki-67 shares 46% aa sequence identity with the mouse ortholog to Ki-67. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma
Authors: J Wang, G He, Q Yang, L Bai, B Jian, Q Li, Z Li
Oncol Lett, 2018;15(6):9009-9016.
Species: Human
Sample Type: Tissue Homogenates
Application: WB
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