抗Human VISTA/B7-H5/PD-1H Affinity Purified Polyclonal Ab抗体(Anti-Human VISTA/B7-H5/PD-1H Affinity Purified Polyclonal Ab antibody)
掲載日情報：2019/10/21 現在Webページ番号：195003
Human VISTA/B7-H5/PD-1H Affinity Purified Polyclonal Abに対する抗体(Anti-Human VISTA/B7-H5/PD-1H Affinity Purified Polyclonal Ab )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月03日 15時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Human VISTA/B7-H5/PD-1H Affinity Purified Polyclonal Ab |
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Anti-Human VISTA/B7-H5/PD-1H Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2024年05月03日 15時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human VISTA/B7-H5/PD-1H Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF7126-100
- メーカー：RSD
- 包装：100μg
- 価格：¥100,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human VISTA/B7-H5/PD-1H Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF7126-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human VISTA/B7-H5/PD-1H Phe33-Ala194Accession # Q9H7M9 |
| Source | Polyclonal Sheep IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human VISTA/B7-H5/PD-1H in direct ELISAs and Western blots. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | See below |
| ELISA | This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB71264). This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human VISTA/B7-H5/PD-1H DuoSet ELISA Kit (Catalog # DY7126-05) for convenient development of a sandwich ELISA. | |
| Flow Cytometry | 0.25 µg/106 cells | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Western Blot | |
|---|---|
 | Detection of Human VISTA/B7-H5/PD-1H by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human VISTA/B7‑H5/PD‑1H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7126) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for VISTA/B7-H5/PD-1H at approximately 45-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Flow Cytometry | |
 | Detection of VISTA/B7-H5/PD-1H in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD11b/Integrin alpha M APC‑conjugated Monoclonal Antibody (Catalog # FAB16991A) and either (A) Sheep Anti-Human VISTA/B7-H5/PD-1H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7126) or (B) Normal Sheep IgG Control (Catalog # 5-001-A) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126). View our protocol for Staining Membrane-associated Proteins. |
| ELISA | |
 | Human VISTA/B7-H5/PD-1H ELISA Standard Curve. Recombinant Human VISTA/B7‑H5/PD‑1H protein was serially diluted 2-fold and captured by Mouse Anti-Human VISTA/B7‑H5/PD‑1H Monoclonal Antibody (Catalog # MAB71264) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human VISTA/B7-H5/PD-1H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7126) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994). |
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Related Product & Information
| Entrez Gene IDs | 64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey) |
|---|---|
| Background | VISTA/B7-H5/PD-1H |
| background_content | Background: VISTA/B7-H5/PD-1H Platelet Receptor Gi24, also known as VISTA (V-domain Ig suppressor of T cell activation), B7-H5, B7H5, Dies1 (Differentiation of ESC-1), SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, VISTA appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, VISTA both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human VISTA contains a 162 aa extracellular region with one V-type Ig-like domain and a 96 aa cytoplasmic domain. Human VISTA undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. Over aa 33-194, human VISTA shares 70% and 67% aa identity with mouse and rat Gi24, respectively. |
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