抗PRDM16抗体(Anti-PRDM16, Sheep-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse PRDM16Lys537-Glu688Accession # A2A935.1
Source	Polyclonal Sheep IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse and human PRDM16 in direct ELISAs and Western blots.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human PRDM16 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse PRDM16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6295) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PRDM16 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunohistochemistry
	PRDM16 in Mouse Embryo. PRDM16 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Sheep Anti-Human/Mouse PRDM16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6295) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (orange, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to the trigeminal ganglion. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

目次に戻る

Related Product & Information

Long Name	PR Domain Containing 16
Background	PRDM16/MEL1
background_content	Background: PRDM16/MEL1 PRDM16 (PR [PRDI-BF1 and RIZ] domain containing protein 16; also MEL-1) is a 170 kDa member of the PR Domain family of proteins. It is a transcriptional regulator expressed in the embryo, and is reported to participate in the maintenance of both neuronal and hematopoietic progenitor stem cells populations, and to preferentially promote the development of brown fat from adipomyocyte precursors. The generation of brown fat is likely due to suppression of muscle-specific factors. Mouse PRDM16 is 1275 amino acids (aa) in length. It contains one SET domain (aa 85-208) followed by ten C2H2 type Zn finger motifs (aa 230-1030). There are multiple potential isoform variants that likely vary from 150-170 kDa in size. One isoform shows a deletion of aa 1232-1250, a second isoform shows a three aa substitution for aa 1174-1275, and a third isoform possesses an alternative start site at Met21, coupled to a deletion of aa 1196-1133. Over aa 537-688, mouse PRDM16 shares 81% and 95% aa identity with human and rat PRDM16, respectively.

目次に戻る

Citations

1. Cbx4 Sumoylates Prdm16 to Regulate Adipose Tissue Thermogenesis
   Authors: Q Chen, L Huang, D Pan, LJ Zhu, YX Wang
   Cell Rep, 2018;22(11):2860-2872.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. Loss of ADAMTS5 enhances brown adipose tissue mass and promotes browning of white adipose tissue via CREB signaling
   Authors: D Bauters, M Cobbaut, L Geys, J Van Lint, B Hemmeryckx, HR Lijnen
   Mol Metab, 2017;6(7):715-724.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


3. Transcription factor Hlx controls a systematic switch from white to brown fat through Prdm16-mediated co-activation
   Authors: L Huang, D Pan, Q Chen, LJ Zhu, J Ou, M Wabitsch, YX Wang
   Nat Commun, 2017;8(1):68.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: IP


4. The Transcription Factor Prdm16 Marks a Single Retinal Ganglion Cell Subtype in the Mouse Retina
   Authors: S Groman-Lup, J Adewumi, KU Park, JA Brzezinski
   Invest. Ophthalmol. Vis. Sci., 2017;58(12):5421-5433.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-Fr


5. Fasting induces a subcutaneous-to-visceral fat switch mediated by microRNA-149-3p and suppression of PRDM16
   Authors: Hanying Ding
   Nat Commun, 2016;7(0):11533.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


6. White-to-brown metabolic conversion of human adipocytes by JAK inhibition.
   Authors: Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C
   Nat Cell Biol, 2015;17(1):57-67.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. MicroRNA-378 controls classical brown fat expansion to counteract obesity.
   Authors: Pan, Dongning, Mao, Chunxiao, Quattrochi, Brian, Friedline, Randall, Zhu, Lihua J, Jung, Dae Youn, Kim, Jason K, Lewis, Brian, Wang, Yong-Xu
   Nat Commun, 2014;5(0):4725.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


8. KSRP ablation enhances brown fat gene program in white adipose tissue through reduced miR-150 expression.
   Authors: Chou C, Lin Y, Wang H, Zhu X, Giovarelli M, Briata P, Gherzi R, Garvey W, Chen C
   Diabetes, 2014;63(9):2949-61.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


9. MyomiR-133 regulates brown fat differentiation through Prdm16.
   Authors: Trajkovski, Mirko, Ahmed, Kashan, Esau, Christin, Stoffel, Markus
   Nat Cell Biol, 2012;14(12):1330-5.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


10. Brown remodeling of white adipose tissue by SirT1-dependent deacetylation of Ppargamma.
    Authors: Qiang L, Wang L, Kon N, Zhao W, Lee S, Zhang Y, Rosenbaum M, Zhao Y, Gu W, Farmer S, Accili D
    Cell, 2012;150(3):620-32.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB

目次に戻る