抗Arginase 1抗体(Anti-Arginase 1, Sheep-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant human Arginase 1/ARG1Met1-Lys322Accession # P05089
Source	Polyclonal Sheep IgG
Purification	Antigen Affinity-purified
Specificity	Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.25 µg/mL	See below
Simple Western	2.5 µg/mL	See below
Immunoprecipitation	25 µg/mL	Cell lysates spiked with Recombinant Human Arginase 1/ARG1 (Catalog # 5868-AR), see our available Western blot detection antibodies

Western Blot
	Detection of Human and Rat Arginase 1/ARG1 by Western Blot. Western blot shows lysates of human liver tissue and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human/Mouse/Rat Arginase 1/ARG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5868) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Arginase 1/ARG1 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Simple Western
	Detection of Human, Mouse, and Rat Arginase 1/ARG1 by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Arginase 1/ARG1 at approximately 41 kDa (as indicated) using 2.5 µg/mL of Sheep Anti-Human/Mouse/Rat Arginase 1/ARG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5868) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Liver-Type Arginase
Background	Arginase 1/ARG1
background_content	Background: Arginase 1/ARG1 Arginase 1 (ARG1) is a 35‑40 kDa member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322 amino acids (aa) in length. Its enzyme region comprises aa 9‑309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105 kDa homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa 204‑289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

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Citations

1. IL-33 contributes to sepsis-induced long-term immunosuppression by expanding the regulatory T cell population
   Authors: DC Nascimento, PH Melo, AR Piñeros, RG Ferreira, DF Colón, PB Donate, FV Castanheir, A Gozzi, PG Czaikoski, W Niedbala, MC Borges, DS Zamboni, FY Liew, FQ Cunha, JC Alves-Filh
   Nat Commun, 2017;8(0):14919.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. Candida albicans Chitin Increases Arginase-1 Activity in Human Macrophages, with an Impact on Macrophage Antimicrobial Functions
   Authors: J Wagener, DM MacCallum, GD Brown, NA Gow
   MBio, 2017;8(1):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Arginase1 Deficiency in Monocytes/Macrophages Upregulates Inducible Nitric Oxide Synthase To Promote Cutaneous Contact Hypersensitivity
   Authors: J Suwanpradi, M Shih, L Pontius, B Yang, A Birukova, E Guttman-Ya, DL Corcoran, LG Que, RM Tighe, AS MacLeod
   J. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Assessment of Preclinical Liver and Skeletal Muscle Biomarkers Following Clofibrate Administration in Wistar Rats
   Authors: P Maliver, M Festag, M Bennecke, F Christen, B Bánfai, B Lenz, M Winter
   Toxicol Pathol, 2017;0(0):1926233177072.
   Species: Rat
   Sample Type: Serum
   Application: ELISA Capture


5. DAMP signaling is a key pathway inducing immune modulation after brain injury.
   Authors: Liesz A, Dalpke A, Mracsko E, Antoine D, Roth S, Zhou W, Yang H, Na S, Akhisaroglu M, Fleming T, Eigenbrod T, Nawroth P, Tracey K, Veltkamp R
   J Neurosci, 2015;35(2):583-98.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB

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