抗Human GABA-A-R beta 3抗体(Anti-Human GABA-A-R beta 3 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human GABAA R beta 3Gln26-Tyr245Accession # P28472
Source	Polyclonal Sheep IgG
Purification	Antigen Affinity-purified
Specificity	Detects human GABAA R beta 3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant rat GABAB R2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Immunohistochemistry
	GABAA R  beta 3 in Human Brain. GABAA R  beta 3 was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human GABAA R  beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5590) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western Blot
	Detection of Human GABAA R  beta 3 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GABAA R  beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5590) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GABAA R  beta 3 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Simple Western
	Detection of Human GABAA R  beta 3 by Simple WesternTM. Simple Western lane view shows lysates of human brain cerebellum tissue, loaded at 0.2 mg/mL. A specific band was detected for GABAA R  beta 3 at approximately 78 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human GABAA R  beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5590) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

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Related Product & Information

Long Name	gamma-Aminobutyric Acid Type A Receptor, beta-3 Polypeptide
Background	GABA-A R beta 3
background_content	Background: GABA-A R beta 3 GABAA R beta 3 (GABA-A receptor subunit beta 3; also GABR3) is a 53-58 kDa member of the ligand-gated ion channel family of proteins. It is generally one of three beta-type subunits that contribute to a GABA-binding pentameric, postsynaptic chloride ion channel on neurons. Alternatively, it can apparently form a homopentameric channel that does not respond to GABA. Mature human GABAA R beta 3 is 448 amino acids (aa) in length. It is a 4-transmembrane glycoprotein that contains an extended N-terminal extracellular region (aa 26-245) and a long cytoplasmic loop between aa 328-450. There are potential splice variants. One shows an alternate start site at Met65, while others contain either a 48 aa substitution for aa 1-228, a nine aa substitution for aa 1-80, or a 26 aa substitution for aa 1-26. Over aa 26-245, human and mouse GABAA R beta 3 are absolutely identical in aa sequence.

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