抗Human SOX11抗体(Anti-Human SOX11 antibody)
掲載日情報：2018/11/26 現在Webページ番号：194507
Human SOX11に対する抗体(Anti-Human SOX11 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2026年07月10日 12時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
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文献数 | ||
|---|---|---|---|---|---|
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Anti-Human SOX11 Affinity Purified Polyclonal Ab |
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本製品は取扱中止になりました | 0 | ||
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Anti-Human SOX11 Affinity Purified Polyclonal Ab |
|
本製品は取扱中止になりました | 0 | ||
[在庫・価格 ：2026年07月10日 12時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human SOX11 Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF3976
- メーカー：RSD
- 包装：100μg
- 本製品は取扱中止になりました
Anti-Human SOX11 Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF3976-SP
- メーカー：RSD
- 包装：25μg
- 本製品は取扱中止になりました
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human SOX11Val2-Pro173Accession # P35716 |
| Source | Polyclonal Sheep IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human SOX11 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) SOX1, rhSOX2, rhSOX3, rhSOX5, rhSOX6, rhSOX7, rhSOX9, rhSOX10, rhSOX12, rhSOX14, rhSOX15, rhSOX17, and rhSOX21 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | See below |
| Immunohistochemistry | 3-15 µg/mL | See below |
| Immunocytochemistry | 5-15 µg/mL | See below |
| Immunocytochemistry | |
|---|---|
 | SOX11 in HeLa Human Cell Line. SOX11 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human SOX11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3976) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Western Blot | |
 | Detection of Human SOX11 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 25 µg of cytoplasmic (Cyto) and 25 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SOX11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3976) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SOX11 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunohistochemistry | |
 | SOX11 in Human Brain. SOX11 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human SOX11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3976) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS020) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
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Related Product & Information
| Background | SOX11 |
|---|---|
| background_content | Background: SOX11 Human SOX11 (SRY box-related gene 11) is a 60-72 kDa (46.6 kDa predicted), group C member of the SOX family of transcription factors. It is 441 amino acids (aa) in length, and contains one HMG box (aa 49-117), two poly-Gly regions (aa 144-214), one poly-Asp domain (aa 223-233) and one poly-Ser region. The HMG box determines DNA and transcriptional coactivator binding, the poly-Ser region lies in a potent transactivating region, and the poly-Asp domain autoinhibits/regulates activities of the other two regions. SOX11 appears early in development in multiple tissues, and impacts neuron, oligodendrocyte and cartilage formation. Human SOX11 is 94% aa identical to mouse SOX11 over aa 1-173. Mouse SOX11, however, is absent a 12 aa poly-Gly sequence that is present in human SOX11 between aa 145-156. Additional band correspondent to the lower molecular weight may represent SOX11 without posttranslational modifications or human SOX4, over aa 38-123 human SOX11 shares 100% aa identity with human SOX4. (1) |
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