抗Human/Mouse/Rat Cyclophilin A抗体(Anti-Human/Mouse/Rat Cyclophilin A antibody)
掲載日情報：2018/11/26 現在Webページ番号：194495
Human/Mouse/Rat Cyclophilin Aに対する抗体(Anti-Human/Mouse/Rat Cyclophilin A )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2025年05月01日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Human/Mouse/Rat Cyclophilin A Affinity Purified PAb |
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Anti-Human/Mouse/Rat Cyclophilin A Affinity Purified PAb |
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[在庫・価格 ：2025年05月01日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human/Mouse/Rat Cyclophilin A Affinity Purified PAb
文献数： 1
- 商品コード：AF3589
- メーカー：RSD
- 包装：100μg
- 価格：¥106,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human/Mouse/Rat Cyclophilin A Affinity Purified PAb
文献数： 0
- 商品コード：AF3589-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human, Mouse, Rat |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human Cyclophilin AMet1-Glu165Accession # P62937 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human, mouse, and rat Cyclophilin A in Western blots. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | See below |
| Simple Western | 5 µg/mL | See below |
| Immunocytochemistry | 5-15 µg/mL | See below |
| Western Blot | |
|---|---|
 | Detection of Human, Mouse, and Rat Cyclophilin A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Cyclophilin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3589) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cyclophilin A at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunocytochemistry | |
 | Cyclophilin A in PANC-1 Human Cell Line. Cyclophilin A was detected in immersion fixed PANC-1 human pancreatic carcinoma cell line using Goat Anti-Human/Mouse/Rat Cyclophilin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3589) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Simple Western | |
 | Detection of Human and Mouse Cyclophilin A by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Cyclophilin A at approximately 25 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Cyclophilin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3589) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. |
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Related Product & Information
| Background | Cyclophilin A |
|---|---|
| background_content | Background: Cyclophilin A Cyclophilin A, also called Peptidyl-prolyl Isomerase A, PPIA, CYPA, and CYPH, was originally characterized for its ability to catalyze the transition between cis- and trans- proline residues critical for proper folding of proteins (1). Cyclophilin is also incorporated into many viruses, including HIV-1, where it has been speculated to be involved in functions such as viral assembly and infectivity (2). The immunosuppressive activity of cyclosporins has been correlated with their ability to form complexes with cyclophilins that inhibit calcineurin phosphatase activity (3) and prevent incorporation of cyclophilin into viral particles (4). The cyclosporin/cyclophilin complex selectively binds and inactivates calcineurin (3, 5), making it a useful inhibitor for studying calcineurin activity. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- The Novel Extracellular Cyclophilin A (CyPA) - Inhibitor MM284 Reduces Myocardial Inflammation and Remodeling in a Mouse Model of Troponin I -Induced Myocarditis.
Authors: Heinzmann D, Bangert A, Muller A, von Ungern-Sternberg S, Emschermann F, Schonberger T, Chatterjee M, Mack A, Klingel K, Kandolf R, Malesevic M, Borst O, Gawaz M, Langer H, Katus H, Fischer G, May A, Kaya Z, Seizer P
PLoS ONE, 2015;10(4):e0124606.
Species: Mouse
Sample Type: Whole Tissue
Application: IF
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