抗Oct3/4抗体(Anti-Oct3/4, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant human Oct‑3/4Met1-Asn265 (Met262Leu)Accession # Q01860
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Oct-3/4 in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	20 µg/mL	See below
Immunoprecipitation	3 µg/106 cells	NTera‑2 human testicular embryonic carcinoma cell line, see our available Western blot detection antibodies
Chromatin Immunoprecipitation (ChIP)	5 µg/106 cells	See below
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human and Mouse Oct‑3/4 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells and D3 mouse embryonic stem cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Oct‑3/4 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chromatin Immunoprecipitation (ChIP)
	Detection of Oct‑3/4-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Oct‑3/4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The nanog promoter was detected by standard PCR.
Immunocytochemistry
	Alkaline Phosphatase and Oct‑3/4 in BG01V Human Stem Cells. Alkaline phosphatase (ALPL) and Oct‑3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Human/Mouse/Rat ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; Catalog # NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western
	Detection of Human Oct‑3/4 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Oct‑3/4 at approximately 50 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Background

Oct-3/4

background_content

Background: Oct-3/4 Oct-3/4, a member of POU transcription factors, was identified as a DNA-binding protein that activates gene transcription via a cis-element containing an octamer motif (1). It is expressed in totipotent embryonic stem and germ cells (2, 3). A critical amount of Oct-3/4 expressed is required to sustain stem cell self-renewal and pluripotency (4). When embryonic stem cells are induced to differentiate, Oct-3/4 is downregulated and this downregulation of Oct-3/4 has proven to be essential for proper and divergent developmental program (5). The Oct-3/4 molecule is not only a master regulator of pluripotency that controls the lineage commitment but also is the most recognized marker used for the identification of totipotent embryonic stem cells.

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Citations

1. Dinaciclib potently suppresses MCL-1 and selectively induces the cell death in human iPS cells without affecting the viability of cardiac tissue
   Authors: K Alsayegh, K Matsuura, H Sekine, T Shimizu
   Sci Rep, 2017;7(0):45577.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9
   Stem Cell Res, 2016;17(2):441-443.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC - Fresh


3. Brain tissue banking for stem cells for our future
   Sci Rep, 2016;6(0):39394.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: IHC Not Specified


4. Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9
   Stem Cell Res, 2016;17(2):430-432.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC Fresh


5. Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells.
   Authors: Wada, Naohisa, Maeda, Hidefumi, Hasegawa, Daigaku, Gronthos, Stan, Bartold, Peter Ma, Menicanin, Danijela, Fujii, Shinsuke, Yoshida, Shinichi, Tomokiyo, Atsushi, Monnouchi, Satoshi, Akamine, Akifumi
   Stem Cells Dev, 2014;23(18):2225-36.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC Fresh


6. Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells.
   Authors: Luo, Lan, Kawakatsu, Miho, Guo, Chao-Wan, Urata, Yoshishi, Huang, Wen-Jing, Ali, Haytham, Doi, Hanako, Kitajima, Yuriko, Tanaka, Takayuki, Goto, Shinji, Ono, Yusuke, Xin, Hong-Bo, Hamano, Kimikazu, Li, Tao-Shen
   Sci Rep, 2014;4(0):3779.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. Pluripotent stem cells derived from mouse and human white mature adipocytes.
   Authors: Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K
   Stem Cells Transl Med, 2014;3(2):161-71.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


8. Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming.
   Authors: Apostolou E, Ferrari F, Walsh R, Bar-Nur O, Stadtfeld M, Cheloufi S, Stuart H, Polo J, Ohsumi T, Borowsky M, Kharchenko P, Park P, Hochedlinger K
   Cell Stem Cell, 2013;12(6):699-712.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: ChIP


9. Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
   Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji
   PLoS ONE, 2012;7(9):e45435.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


10. A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells.
    Authors: Wong RC, Pollan S, Fong H, Ibrahim A, Smith EL, Ho M, Laslett AL, Donovan PJ
    Stem Cells, 2011;29(10):1517-27.
    Species: Human
    Sample Type: Cell Lysates
    Application: ChIP

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