抗HMGB1抗体(Anti-HMGB1, Chicken-Poly antibody)
掲載日情報:2018/11/26 現在Webページ番号:194454
HMGB1に対する抗体(Anti-HMGB1, Chicken-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 :2025年05月28日 00時01分現在]
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Anti-HMGB1, Chicken-Poly |
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Anti-Human/Mouse HMGB1 Affinity Purified Polyclonal Ab |
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[在庫・価格 :2025年05月28日 00時01分現在]
Anti-HMGB1, Chicken-Poly
文献数: 5
- 商品コード:AF1690
- メーカー:RSD
- 包装:100μg
- 価格:¥105,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 別名:Amphoterin Genbank No: 3146 Protein Accession No: P09429.3 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Chicken | |||||
交差性 | Human | 適用 | Western Blot | ||||
標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
吸収処理 | クラス | IgY | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 抗HMGB1 / HMG-1抗体 |
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関連記事 |
Anti-Human/Mouse HMGB1 Affinity Purified Polyclonal Ab
文献数: 1
- 商品コード:AF1690-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
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Product Details
Species Reactivity | Human, Mouse |
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Label | Unconjugated |
Immunogen | Mouse myeloma cell line NS0-derived recombinant human HMGB1/HMG‑1Met1-Glu215Accession # P09429.3 |
Source | Polyclonal Chicken IgY |
Purification | Antigen Affinity-purified |
Specificity | Detects human and mouse HMGB1/HMG‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) HMGB3 is observed, and less than 1% cross-reactivity with rhHMGA1, rhHMGA2, and rhHMGA1b is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 1 µg/mL | See below |
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Related Product & Information
Background | HMGB1/HMG-1 |
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background_content | Background: HMGB1/HMG-1 Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins (1-3). Human HMGB1 is expressed as a 25-30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu (4, 5). Residues 27-43 and 178-184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues (6). HMGB1 is expressed at high levels in almost all cells (2, 4). It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins (1, 7, 8). It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction (1, 4, 7, 9, 10). The cytokine activity of HBMG1 is restricted to the HMG B box, (3) while the A box is associated with the helix-loop-helix domain of transcription factors (11). HMBG1 is released in response to cell death and as a secretion product. Although HMBG-1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis (6, 12). Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4 (1, 3, 4). Human HMGB1 is 100% aa identical to canine HMGB1 and 99% aa identical to mouse, rat, bovine and porcine HMGB1, respectively. |
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Citations
- Cancer usurps skeletal muscle as an energy repository.
Authors: Luo, Yi, Yoneda, Junya, Ohmori, Hitoshi, Sasaki, Takamits, Shimbo, Kazutaka, Eto, Sachise, Kato, Yumiko, Miyano, Hiroshi, Kobayashi, Tsuyoshi, Sasahira, Tomonori, Chihara, Yoshitom, Kuniyasu, Hiroki
Cancer Res, 2014;74(1):330-40.
Species: Mouse
Sample Type: In Vivo
Application: Neut - Sputum biomarkers and the prediction of clinical outcomes in patients with cystic fibrosis.
Authors: Liou T, Adler F, Keogh R, Li Y, Jensen J, Walsh W, Packer K, Clark T, Carveth H, Chen J, Rogers S, Lane C, Moore J, Sturrock A, Paine R, Cox D, Hoidal J
PLoS ONE, 2012;7(8):e42748.
Species: Human
Sample Type: Sputum
Application: ELISA Development
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