抗TNF Receptor Ⅱ抗体(Anti-TNF Receptor Ⅱ, Soluble, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse TNF RII/TNFRSF1BVal23-Gly258Accession # P25119
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse TNF RII in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TNF RII is observed and less than 1% cross-reactivity with recombinant mouse (rm) CD27, rmGITR, rhDR6, rmCD30, rmCD40, rmEDAR, rmFas, rmOPG, rmRANK, and rmTNF RI is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
ELISA	This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB4263). This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse sTNF RII/TNFRSF1B DuoSet ELISA Kit (Catalog # DY426) for convenient development of a sandwich ELISA or the Mouse/Rat TNF RII/TNFRSF1B Quantikine ELISA Kit (Catalog # MRT20) for a complete optimized ELISA.
Flow Cytometry	2.5 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	Immersion fixed mouse splenocytes

Western Blot
	Detection of Mouse TNF RII/TNFRSF1B by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 1 µg/mL LPS for 24 hours. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TNF RII/TNFRSF1B at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Flow Cytometry
	Detection of TNF RII/TNFRSF1B in L‑929 Mouse Cell Line by Flow Cytometry. L‑929 mouse fibroblast cell line was stained with Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

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Related Product & Information

Long Name	Tumor Necrosis Factor Receptor II
Entrez Gene IDs	7133 (Human); 21938 (Mouse); 156767 (Rat)
Background	TNF RII/TNFRSF1B
background_content	Background: TNF RII/TNFRSF1B Two types of soluble TNF receptors have been identified in human serum and urine which can neutralize the biological activities of TNF-alpha and TNF-beta. These binding proteins represent truncated forms of the two types of high-affinity cell surface receptors for TNF (TNFR-p60 Type B and TNFR-p80 Type A). Soluble TNF RII corresponds to TNFR-p80 Type A. In the new TNF superfamily nomenclature, TNF RII is referred to as TNFRSF1B. These apparent soluble forms of the receptors appear to arise as a result of shedding of the extracellular domains of the membrane-bound receptors. Normal concentrations as high as 4 ng/mL are found in the serum of healthy individuals, and even higher levels may be found in some pathological conditions. Although the physiological role of these proteins is not known, it has been speculated that shedding of the soluble receptors in response to TNF release could serve as a mechanism to scavenge the TNF not immediately bound and thus localize the inflammatory response. It is also possible that the pool of TNF bound to soluble receptors could represent a reservoir for the controlled release of TNF.

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Citations

1. Endogenous TNF? orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation
   Authors: S Arokiasamy, C Zakian, J Dilliway, W Wang, S Nourshargh, MB Voisin
   Sci Rep, 2017;7(0):44189.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2
   Authors: R Fischer, J Marsal, C Guttà, SA Eisler, N Peters, JR Bethea, K Pfizenmaie, RE Kontermann
   Sci Rep, 2017;7(1):6607.
   Species: Mouse
   Sample Type: Whole Cells
   


3. Phenotypic screening reveals TNFR2 as a promising target for cancer immunotherapy
   Oncotarget, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Myosin light chain kinase expression induced via tumor necrosis factor receptor 2 signaling in the epithelial cells regulates the development of colitis-associated carcinogenesis.
   Authors: Suzuki M, Nagaishi T, Yamazaki M, Onizawa M, Watabe T, Sakamaki Y, Ichinose S, Totsuka M, Oshima S, Okamoto R, Shimonaka M, Yagita H, Nakamura T, Watanabe M
   PLoS ONE, 2014;9(2):e88369.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


5. Therapy of peritoneally disseminated colon cancer by TAP-deficient embryonic stem cell-derived macrophages in allogeneic recipients.
   Authors: Haga E, Endo Y, Haruta M, Koba C, Matsumura K, Takamatsu K, Ikeda T, Nishimura Y, Senju S
   J Immunol, 2014;193(4):2024-33.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


6. Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
   Authors: Proebstl D, Voisin MB, Woodfin A, Whiteford J, D'Acquisto F, Jones GE, Rowe D, Nourshargh S
   J. Exp. Med., 2012;209(6):1219-34.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
   Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
   J. Clin. Invest., 2009;119(10):2954-64.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen

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