抗FCRN抗体(Anti-FCRN, Mouse-Mono antibody)
掲載日情報：2018/11/26 現在Webページ番号：187772
FCRNに対する抗体(Anti-FCRN, Mouse-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2025年05月22日 13時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
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Anti-FCRN, Mouse-Mono(937508) |
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Anti-FCRN, Mouse-Mono(937508) |
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[在庫・価格 ：2025年05月22日 13時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-FCRN, Mouse-Mono(937508)
文献数： 0
- 商品コード：MAB8639-100
- メーカー：RSD
- 包装：100μg
- 価格：¥104,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-FCRN, Mouse-Mono(937508)
文献数： 0
- 商品コード：MAB8639-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human FCRNAla24-Ser297Accession # P55899 |
| Source | Monoclonal Mouse IgG2B Clone # 937508 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human FCRN in direct ELISAs. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 5 µg/mL | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Intracellular Staining by Flow Cytometry | 0.25 µg/106 cells | See below |
| Western Blot | |
|---|---|
 | Detection of Human FCRN by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human FCRN Monoclonal Antibody (Catalog # MAB8639) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FCRN at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. |
| Intracellular Staining by Flow Cytometry | |
 | Detection of FCRN in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human FCRN Monoclonal Antibody (Catalog # MAB8639, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules. |
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Related Product & Information
| Background | FCRN |
|---|---|
| background_content | Background: FCRN The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells (1, 2). Mature human FCRN consists of a 274 amino acid (aa) extracellular domain (ECD) with two N-terminal alpha domains, one alpha 3/immunoglobulin-like domain, a 23 aa transmembrane segment, and a 44 aa cytoplasmic domain (3). Within the ECD, human FCRN shares 68% aa sequence identity with mouse and rat FCRN. Mouse FCRN binds with high affinity to IgG from mouse, human, rat, rabbit, guinea pig, bovine, and sheep, while human FCRN binds IgG with significantly lower affinity and is much more restricted in terms of species recognition (4). It does not bind the structurally related chicken IgY (5). FCRN additionally binds to albumin, and both it and IgG are bound at pH 5.0 but not at pH 8.0 (3, 6). FCRN associates noncovalently with beta 2-Microglobulin, and this interaction is important for the intracellular trafficking of FCRN (7-10). FCRN cycles between the plasma membrane and acidified intracellular compartments of endothelial cells and epithelial cells (5, 8). It binds endocytosed IgG and albumin in the low pH vesicles and transports them to the plasma membrane for extracellular release at higher pH. This protects IgG and albumin from lysosomal degradation and helps maintain the circulating levels of both proteins (5, 6). This mechanism is involved in the bidirectional transport of IgG across epithelial and endothelial barriers including neonatal IgG absorption in the intestine and fetal uptake of maternal antibodies through the placenta (5, 8, 11, 12). In the kidney, FCRN recycles albumin to the serum but removes IgG from the glomular basement membrane and promotes its excretion into the urine (13, 14). FCRN is also expressed in neutrophils and myeloid antigen presenting cells (7, 15, 16). It can enhance IgG-mediated phagocytosis and antigen presentation by these cells, but it promotes the degradation of opsonizing IgG rather than returning it to the circulation (15, 16). |
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