抗Mouse CXCR2抗体(Anti-Mouse CXCR2 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	C6 rat glioma cell line transfected with mouse CXCR2Met1-Leu359Accession # P35343
Source	Monoclonal Rat IgG2A Clone # 242216
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Stains mouse CXCR2 transfected cells but not CXCR1 transfectants.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-reported	Wang, G. et al. (2016) Cancer Discov. 6: 80. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization	Measured by its ability to neutralize CXCL2/GRO beta /MIP‑2/CINC‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR2. The Neutralization Dose (ND50) is typically 15-50 µg/mL in the presence of 2 ng/mL Recombinant Mouse CXCL2/GRO beta /MIP‑2/CINC‑3.

Neutralization
	Chemotaxis Induced by CXCL2/MIP‑2 and Neutralization by Mouse CXCR2/IL‑8 RB Antibody. Recombinant Mouse CXCL2/MIP‑2 (Catalog #452-M2) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB2164). The ND50 is typically 15-50 µg/mL.
Flow Cytometry
	Detection of CXCR2/IL‑8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouseCXCR1/IL-8 RA  and eGFP was stained with Rat Anti-Mouse CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB2164) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006).

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Related Product & Information

Long Name	Interleukin 8 Receptor B
Entrez Gene IDs	3579 (Human); 12765 (Mouse); 29385 (Rat)
Background	CXCR2/IL-8 RB
background_content	Background: CXCR2/IL-8 RB CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.

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Citations

1. Novel adherent CD11b+Gr-1+tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment
   Authors: T Tsubaki, T Kadonosono, S Sakurai, T Shiozawa, T Goto, S Sakai, T Kuchimaru, T Sakamoto, H Watanabe, G Kondoh, S Kizaka-Kon
   Oncotarget, 2018;9(13):11209-11226.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Microbiome-mediated neutrophil recruitment via CXCR2 and protection from amebic colitis
   Authors: K Watanabe, CA Gilchrist, MJ Uddin, SL Burgess, MM Abhyankar, SN Moonah, Z Noor, JR Donowitz, BN Schneider, T Arju, E Ahmed, M Kabir, M Alam, R Haque, P Pramoonjag, B Mehrad, WA Petri
   PLoS Pathog., 2017;13(8):e1006513.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


3. The mTOR signal regulates myeloid-derived suppressor cells differentiation and immunosuppressive function in acute kidney injury
   Authors: C Zhang, S Wang, J Li, W Zhang, L Zheng, C Yang, T Zhu, R Rong
   Cell Death Dis, 2017;8(3):e2695.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. The calcineurin-NFAT axis controls allograft immunity in myeloid-derived suppressor cells through reprogramming T cell differentiation.
   Authors: Wang X, Bi Y, Xue L, Liao J, Chen X, Lu Y, Zhang Z, Wang J, Liu H, Yang H, Liu G
   Mol Cell Biol, 2015;35(3):598-609.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


5. Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy.
   Authors: Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K
   J Biol Chem, 2015;290(4):2053-68.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


6. IL-17A produced by neutrophils protects against pneumonic plague through orchestrating IFN-gamma-activated macrophage programming.
   Authors: Bi Y, Zhou J, Yang H, Wang X, Zhang X, Wang Q, Wu X, Han Y, Song Y, Tan Y, Du Z, Yang H, Zhou D, Cui Y, Zhou L, Yan Y, Zhang P, Guo Z, Wang X, Liu G, Yang R
   J Immunol, 2014;192(2):704-13.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


7. Phosphatase Wip1 negatively regulates neutrophil migration and inflammation.
   Authors: Sun B, Hu X, Liu G, Ma B, Xu Y, Yang T, Shi J, Yang F, Li H, Zhang L, Zhao Y
   J Immunol, 2014;192(3):1184-95.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


8. Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments.
   Authors: Sprague L, Muccioli M, Pate M, Singh M, Xiong C, Ostermann A, Niese B, Li Y, Li Y, Courreges M, Benencia F
   Exp Cell Res, 2014;323(1):7-27.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


9. Platelets guide the formation of early metastatic niches.
   Authors: Labelle, Myriam, Begum, Shahinoo, Hynes, Richard
   Proc Natl Acad Sci U S A, 2014;111(30):E3053-61.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


10. mTOR limits the recruitment of CD11b+Gr1+Ly6Chigh myeloid-derived suppressor cells in protecting against murine immunological hepatic injury.
    Authors: Zhang Y, Bi Y, Yang H, Chen X, Liu H, Lu Y, Zhang Z, Liao J, Yang S, Chu Y, Yang R, Liu G
    J Leukoc Biol, 2014;95(6):961-70.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo

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