抗Mouse B7-H1/PD-L1 Alexa Fluor 647抗体(Anti-Mouse B7-H1/PD-L1 Alexa Fluor 647 antibody)

掲載日情報:2018/11/26 現在Webページ番号:186796

Mouse B7-H1/PD-L1 Alexa Fluor 647に対する抗体(Anti-Mouse B7-H1/PD-L1 Alexa Fluor 647 )です。
本製品は研究用です。研究用以外には使用できません。

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Anti-Mouse B7-H1/PD-L1 Alexa Fluor 647 MAb (Clone 929903)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
別名:B7-H
クローン:929903
Genbank No: 29126
Protein Accession No: Q9EP73
法規制等
保存条件 法規備考
抗原種 免疫動物 Rat クラス IgG 標識 Alexa Fluor 647
交差性 Mouse 適用 FCM
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
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[在庫・価格 :2024年05月03日 00時01分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Mouse B7-H1/PD-L1 Alexa Fluor 647 MAb (Clone 929903)

文献数: 0

説明文 別名:B7-H
クローン:929903
Genbank No: 29126
Protein Accession No: Q9EP73
法規制等
保存条件 法規備考
抗原種 免疫動物 Rat
交差性 Mouse 適用 FCM
標識 Alexa Fluor 647 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事
関連記事



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Product Details

Species ReactivityMouse
LabelAlexa Fluor 647
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse B7-H1/PD-L1Met1-Thr239Accession # Q9EP73
SourceMonoclonal Rat IgG2A Clone # 929903
PurificationProtein A or G purified from hybridoma culture supernatant
SpecificityDetects mouse B7-H1/PD-L1 in direct ELISAs. Stains mouse B7-H1/PD-L1 transfectants but not irrelevant transfectants in flow cytometry.


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Applications and Data

 Recommended
Concentration
Sample
Flow Cytometry5 µL/106 cellsSee below


Flow Cytometry
Detection of B7‑H1/PD‑L1 in HEK293 Human Cell Line Transfected with Mouse B7-H1 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) mouse B7-H1 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse B7‑H1/PD‑L1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB9078R). Quadrant markers were set based on control antibody staining (Catalog # IC006R). View our protocol for Staining Membrane-associated Proteins.


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Related Product & Information

BackgroundPD-L1/B7-H1
background_contentBackground:
PD-L1/B7-H1
Mouse B7 homolog 1(B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1‑4). Other family members include B7-1, B7-2, B7-H2, B7-H3 and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and a short cytoplasmic region. Among the family members, they share from 20‑40% amino acid (aa) sequence identity. The cloned mouse B7-H1/PD-L1 cDNA encodes a 290 aa type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular region containing one V-like and one C-like Ig domain, a 22 aa transmembrane region, and a 30 aa cytoplasmic domain. Mouse and human B7-H1/PD-L1 share approximately 70% aa sequence identity. B7-H1/PD-L1 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L2. Mouse B7-H1/PD-L1 and PD-L2 share approximately 34% aa sequence identity and have similar functions. B7-H1/PD-L1 is constitutively expressed in various lymphoid and non-lymphoid organs including placenta, heart, pancreas, lung, liver, and endothelium (1‑4). The expression of B7-H1/PD-L1 is detected on B cells, T cells, monocytes, dendritic cells and thymic epithelial cells. IFN-gamma treatment induces B7-H1/PD-L1 expression in monocytes, dendritic cells, and endothelial cells. B7-H1/PD-L1 expression is also upregulated in a variety of tumor cell lines. On previously activated T cells, B7-H1/PD-L1 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a costimulatory function for the PD-1 ligands on resting T cells has also been reported (1‑4).


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