抗Human CD1c Allophycocyanin抗体(Anti-Human CD1c Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Allophycocyanin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human CD1c/BDCA‑1Ala19-Met302 (Phe300Ser)Accession # P29017
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CD1c in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) CD1a, rhCD1b, rhCD1d, and rhCD1e is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of CD1c/BDCA‑1 in MOLT‑4 Human Cell Line by Flow Cytometry. MOLT‑4 human acute lymphoblastic leukemia cell line was stained with Goat Anti-Human CD1c/BDCA‑1 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5910A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Entrez Gene IDs	911 (Human)
Background	CD1c/BDCA-1
background_content	Background: CD1c/BDCA-1 CD1c (Cluster of Differentiation antigen 1c) is a 43 kDa member of the CD1 family of molecules. It is expressed by thymocytes, dendritic cells and B cells, and exists as part of a noncovalent complex with 12 kDa beta 2‑microglobulin. It is found in the plasma membrane and early endosomes (but not lysosomes), and is presumed to present glycolipids and acylated peptides to T cells. Mature human CD1c is a 316 amino acid (aa) type I transmembrane glycoprotein. It contains a 285 aa extracellular domain (ECD) (aa 18‑302) plus a 10 aa cytoplasmic tail. The ECD shows one Ig‑like domain (aa 203‑296) that associates with beta 2‑microglobulin, and a TyrGlnAspIle internalization motif in the cytoplasmic tail. There are three potential splice variants. One shows a Trp substitution for aa 327‑333, a second shows an eight aa substitution for aa 298‑333, and a third shows a 50 aa substitution for aa 297‑333. There appears to be no direct mouse counterpart to human CD1c.

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Citations

1. Residual endotoxin contaminations in recombinant proteins are sufficient to activate human CD1c+ dendritic cells.
   Authors: Schwarz H, Schmittner M, Duschl A, Horejs-Hoeck J
   PLoS ONE, 2014;9(12):e113840.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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