抗Human/Mouse/Rat beta-Actin抗体(Anti-Human/Mouse/Rat beta-Actin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	Peptide containing a sequence at the N-terminus of human beta ‑ActinAccession # P60709
Source	Monoclonal Mouse IgG1 Clone # 937215
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human, mouse, and rat  beta ‑Actin in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.01 µg/mL	See below
Simple Western	1 µg/mL	See below
Immunocytochemistry	0.1-25 µg/mL	See below

Western Blot
	Detection of Human, Mouse, and Rat beta ‑Actin by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.01 µg/mL of Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody (Catalog # MAB8929) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta ‑Actin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	beta ‑Actin in NIH‑3T3 Mouse Cell Line. beta ‑Actin was detected in immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody (Catalog # MAB8929) at 0.1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Cells were fixated in methanol. Specific staining was localized to cytoskeleton.
Simple Western
	Detection of Human beta ‑Actin by Simple WesternTM. Simple Western lane view shows lysate of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for beta ‑Actin at approximately 49 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody (Catalog # MAB8929). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Entrez Gene IDs	60 (Human); 11461 (Mouse); 81822 (Rat)
Background	beta-Actin
background_content	Background: beta-Actin Actin makes up the most abundant protein in many eukaryotic cell types. It polymerizes to form microfilaments that have an array of functions, including regulating contractility, motility, cytokinesis, phagocytosis, adhesion, cell morphology, and providing structural support. At least six types of Actin, falling into three categories, exist in higher eukaryotes. The three types, separated based on isoelectric point, include alpha ( alpha -Skeletal, alpha -Cardiac, alpha -Smooth Muscle), beta ( beta -Non-muscle) and gamma ( gamma -Smooth Muscle, gamma -non-muscle) isoforms.  Non-muscle beta - and gamma -actin are also known as cytoplasmic actin.

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Citations

1. B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma
   Authors: J Wang, G He, Q Yang, L Bai, B Jian, Q Li, Z Li
   Oncol Lett, 2018;15(6):9009-9016.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


2. DNA methylation affects metastasis of renal cancer and is associated with TGF-?/RUNX3 inhibition
   Authors: J Zheng, Y Mei, P Xiang, G Zhai, N Zhao, C Xu, M Liu, Z Pan, K Tang, D Jia
   Cancer Cell Int., 2018;18(0):56.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Association between circadian gene CLOCK and cisplatin resistance in ovarian cancer cells: A preliminary study
   Authors: H Xu, Z Wang, G Mo, H Chen
   Oncol Lett, 2018;15(6):8945-8950.
   Species: Human
   Sample Type: Whole Cells
   Application: WB


4. Cytoprotective Effects and Mechanisms of ?-17 Fatty Acid Desaturase in Injured Human Umbilical Vein Endothelial Cells (HUVECs)
   Authors: H Zhou, C Wang
   Med. Sci. Monit., 2017;23(0):1627-1635.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Pharmacological inhibition of macrophage toll-like receptor 4/nuclear factor-kappa B alleviates rhabdomyolysis-induced acute kidney injury
   Authors: RS Huang, JJ Zhou, YY Feng, M Shi, F Guo, SJ Gou, S Salerno, L Ma, P Fu
   Chin. Med. J., 2017;0(0):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


6. Hochu-ekki-to Treatment Improves Reproductive and Immune Modulation in the Stress-Induced Rat Model of Polycystic Ovarian Syndrome
   Authors: E Park, CW Choi, SJ Kim, YI Kim, S Sin, JP Chu, JY Heo
   Molecules, 2017;22(6):.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: WB


7. Anti-neoplastic drugs increase caveolin-1-dependent migration, invasion and metastasis of cancer cells
   Authors: NI Díaz-Valdi, CC Calderón, JE Díaz, L Lobos-Gonz, H Sepulveda, RJ Ortíz, S Martinez, V Silva, HJ Maldonado, P Silva, S Wehinger, VA Burzio, VA Torres, M Montecino, L Leyton, AFG Quest
   Oncotarget, 2017;8(67):111943-111965.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Microgravity Induction of TRAIL Expression in Preosteoclast Cells Enhances Osteoclast Differentiation
   Sci Rep, 2016;6(0):25143.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


9. Fatty acid-binding protein E-FABP restricts tumor growth by promoting IFN-beta responses in tumor-associated macrophages.
   Authors: Zhang Y, Sun Y, Rao E, Yan F, Li Q, Zhang Y, Silverstein K, Liu S, Sauter E, Cleary M, Li B
   Cancer Res, 2014;74(11):2986-98.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


10. Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy.
    Authors: Wang Y, Wei Q, Liu Q, Li Z, Zhou L, Zou F, Yuan Y, Sun Z
    Mol Med Rep, 2013;8(6):1817-23.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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